Pipette And Micropipette (Mathematical Skills) Flashcards
In a P20 micropipette, how would you set the pipette to 13 ul?
130
In a P1000, if the micropipette is set at 025, how many ul is that? And ml?
250; 0.250
In a P200 micropipette, how would you set the pipette to 180 ul?
180
In a P1000, if the micropipette is set at 010, how many ul is that? And ml?
100 ul; 0.100 ml
20 ul = ___ ml = ____ g
.02; .02
How do you calculate % error?
(Actual # - Expected #) / (Expected #) * 100
A volume of 4.2L is equivalent to what volume in mL?
4200 mL
Report a volume of 736 uL in units of mL.
0.736 mL
To withdraw the sample from a microcentrifuge, depress the plunger to the _______ and hold it in this position.
First stop
If you notice air space at the end of the tip or air bubbles within the sample in the tip, carefully _____.
expel the sample back into its microcentrifuge tube
To expel the sample into the reaction tube, slowly depress the plunger to the first stop to expel the sample. Then ____.
depress to second stop to blow out the last bit of fluid
To prevent cross-contamination of reagents, remember to
1- Always add a single reagent to reaction tubes;
2- Release each reagent drop onto a new location of the inside wall, near the bottom of the reaction tube;
3- Use a fresh tip for each new reagent to be pipetted;
4- If the tip becomes contaminated, switch to a new one
How do you find the average error?
Take the average of all the errors.
A scientist determines that a sample has a mass of 127 mg. What is the mass of the sample in ug? g? kg?
127,000 ug; 0.127 g; 0.000127 kg
What is the concentration of a 36 uM pyruvate solution, in units of mM?
0.036 mM
if a phosphate buffer has a concentration of 5 mM, what is its concentration in umol/mL?
5 umol/mL
The molecular weights of sucrose and water are 342 gmol^-1 and 18.0 gmol^-1, respectively. 17.3 g of sucrose is dissolved to a final total volume of 246 mL.
a) Write the equation to find the total mol of sucrose used.
b) What is the molar concentration (mol*L^-1) of sucrose in the resulting solution?
a) 342g / 1 mol = 17.3g / x
b) Molar of conc. of sucrose = x / 0.246L
What is M?
mol/L
What is mol*L^-1
mol/L = M
A 10 mL volume of a 5 mM lactic acid solution is mixed with 5 mL of water. Write the equation to find the concentration of the resulting mixture in uM.
(5000 uM)*(10 mL) = C_2 * (10 mL + 5 mL)
1 ml = ___ ul
1000
10 mM = __ uM
10000
A 3 mL volume of a solution containing an unknown concentration of HCl is added to 7 mL of water. It is then determined that the concentration of the HCl in the diluted solution is 630 mM. Write an equation to find the concentration of HCl in the original solution?
(3 mL) * [HCl] = (10 mL) * (630 mM)
Suppose you need 100 mL of a 0.15 M solution of NaCl.
a) Write equation to find the # of mol you need of NaCl.
b) Write the equation to find the mass needed for NaCl.
a) 0.15 mol / 1L = x / 0.1L
b) 58.44 g / mol * x = mass of NaCl