GMO Lab Part II Flashcards
Immediately after PCR amplification, can you determine whether or not you have PCR products?
No.
To visualize your PCR products, what method/tool will you use?
Gel electrophoresis
Your PCR product bands are very ____ compared to those in other DNA experiments you may have done.
small
What is needed to impede the movement of these bands so that it is separated more on the gel and can be seen?
Tighter gel matrix
How much agarose is needed to use agarose electrophoresis?
3%
What is the alternative gel instead of agarose gel?
polyacrylamide gel
What is used to separate smaller molecules for visualization?
PAGE: polyacrylamide gel electrophoresis
What reference will you use to determine the product bands’ sizes?
molecular weight ruler (DNA standard)
After the molecular weight ruler is loaded, what stain will be used to stain the gel?
Fast Blast stain
Why will Fast Blast stain be used to stain the gel?
To make the bands visible.
What is the absorbance of PMM?
455 bp
What is the absorbance of GMO?
200 bp
