Pipets Flashcards
Type I classification that uses water for calibration and allows the flow of liquid by gravity.
Class A: To deliver (TD) pipet
Type I classification is in accordance with which characteristic?
Design
Type I classification B: To contain pipet (TC) uses which substance for calibration?
Hg
What is the function of Type I class B design?
The deliverance of exact volume by repeated filling and emptying of diluents.
Type II classification is in accordance with ______ characteristic.
Drainage
Modified TRUE or FALSE, if FALSE look for the incorrect word/words:
To blow out pipet is similar to TD pipet but needs the last drop to be sucked in to get the exact volume. Calibration is indicated by an etched or frosted rectangle.
False. Sucked in -> blown out. Rectangle -> ring
This type II characteristic allows contents to be drained by gravity.
Self draining
Type III classifications. (2)
Measuring or Graduated pipets and Transfer pipets
This type of class III pipet is usually uniformed graduation in length. It is used to deliver fractional quantity of liquid and is also used for reagent measurements.
Measuring or Graduated pipet
This Type III class A pipet has calibration marks at the tip and is generally also a type II class A pipet.
Serologic
This type III class A pipet does not have graduations to the tip and is also a type II class B pipet.
Mohr
What is a micropipette?
May either be a serological or mohr. Calibrated either TC or TD with total holding volume of less than 1ml
What is the function of type III class B: transfer pipets?
They are designed to dispense one volume
Modified TRUE or FALSE, if FALSE look for the incorrect word/words:
The volumetric pipet (or transfer pipet) has a cylindrical bulb located midway between the mouth and the tip.
True
This is used when measuring non-viscous fluids. It offers the greatest accuracy and precision.
Volumetric or transfer pipets
What is the difference between an Oswald-Folin pipet and a volumetric pipet?
Volumetric pipets have bulbs located at the middle while Oswald-Folin pipets have their bulbs much more proximal to the tip. Volumetric pipets are used to measure non-viscous fluid while OS is used to measure viscous fluid. Volumetric pipets are allowed to drain only while OS pipets are blow-out.
This pipet has no calibration marks and are used to transfer biologic fluids without consideration of specific volume.
Pasteur pipet
What are the advantages of using an automatic pipet?
Safety, stability, ease of use, increased precision, saves time, less cleaning.
The difference between autonomic and micropipet.
Micropipet - less than 1 ml, autonomic - greater than 1 ml
What are the types of autonomic pipets? (3)
Air displacement, positive displacement, dispensers and dilutors.
This type of autonomic pipet relies on a piston for suction creation to draw the sample into a disposable tip that must be changed after each use.
Air displacement
Modified TRUE or FALSE, if FALSE look for the incorrect word/words:
Positive displacement autonomic pipets is operated by moving the piston in the pipet tip or barrel, much like a hypodermic syringe. Requires a different tip after each use. Does not require rinsing and blotting between samples to prevent carryover.
False. Does not require different tip for each use.
Autonomic pipets that obtain the liquid from a common reservoir and dispenses it repeatedly.
Dispensers and dilutors
The dispensing pipets may be _______ ________ ________ _______ to a dilutor
Bottle - top, motorized, handheld, or attached
What are the specifications printed on the neck of the pipet?
Maximum volume, size, temperature, TD or TC
Modified TRUE or FALSE, if FALSE look for the incorrect word/words:
Only blow out has a serological pipet if it has a frosted brand or two thin rings.
True
