Cleaning Techniques

Question 0

To what temperature should the oven be in a routine wash?

Answer 0

Less than 140C

Question 1

Where should dirty glasswares be immersed in during a routine wash?

Answer 1

Soapy water or dilute bleach solution.

Question 2

Which reagents are used in routine washing?

Answer 2

Acid dichromate, 20% nitric acid (washed with detergent and tap water)

Question 3

What technique is used when blood clot is present?

Answer 3

Addition of 10%NaOH with the use of dry micropipets and acetone.

Question 4

How would you clean new pipets? (SI Alkaline)

Answer 4

Rinse with 5% hypochloric acid or 5% nitric acid. Wash with routine procedure.

Question 5

Acid soak (20% nitric acid) for 12-24 hours. Rinse with distilled water 3-4 times.

Answer 5

Metal ion concentration

Question 6

How do you clean grease?

Answer 6

Soak in an organic solvent: dissolve 100g of KOH in 100ml dist. Water. Cool. Add 10% ethanol.

Question 7

Permanganate stains are cleaned used which type of acid?

Answer 7

50% HCl.

Question 8

1% Ferrous sulfate dissolved in 25% sulfuric acid is prepared in which type of cleaning?

Answer 8

Permanganate stains.