Pictures from Class

Question 1

Describe what is happening.

Answer 1

This is SERRATIUS MARCESSENS and the red pigment is PERDIGIOSIN. That is the normal metabolic pathway. The white colonies are a result of UV light and a disruption in this pathway. There are more colonies on the light plate because of PHOTOREACTIVATION.

Question 2

Which organism is positive for chemotaxis?

Answer 2

Bascillus subtilus is (+) for chemotaxis.

Question 3

Describe this.

Answer 3

The E. Coli is most succeptable to UV light. The Bascillus subtilus 24hr culture is more resistant because of endospores. The 7day culture is the most resistant because it has more endopores than thr 24hr culture.

Question 4

Describe this.

Answer 4

The plate on the right is STARCH AGAR. Add Gram’s Iodine and the microbes that are (+) for starch hydrolysis have a clear zone around the growth.

The plate on the left is GELATIN AGAR. Organisms with a clear zone around the growth are positive for gelatin hydrolysis.

Question 5

Motility.

Answer 5

The organism on the left is motile. This was done by a motility stab. You can tell becuase the microbe has grown out from the original stab.

Question 6

Thioglycollate broth

Answer 6

The organism on the LEFT is an OBLIGATE AEROBE. You can tell because all the growth is at the top (pellicle).

The organism on the RIGHT is an OBLIGATE ANAEROBE. You can tell because the growth is at the bottom, away from the oxygen (sediment).

Question 7

Vogues-Proskauer Test Results

Answer 7

The VP(+) is red after 60min.

The VP(-) does not change color.

Question 8

Oxidative/Fermentive Results

Answer 8

Oxidative:

without oil = yellow on the top

with oil = green throughout

Fermentive:

yellow in both

Neither:

without oil: blue

with oil = green

Question 9

Pseudosel Result

Answer 9

Pseudosel (+) has green and yellow.

green = pyocyanin
yellow = flourescine

Selective for Pseudomonas aeruginosa.

Question 10

Urease, Cuitrate, and Phenylalanine Deaminase Test Results

Answer 10

Urease:

(+) pink

(-) yellowish

Citrate:

(+) blue

(-) green

Phenylalanine Demainase:

(+) blue ring around top

(-) none

Question 11

Nitrate Reductase Results

Answer 11

Nitrite (+) = blue

N2 (+) = bubbles in durham tube

(-) nothing

Question 12

Kligler’s Iron Agar Results

Answer 12

LACTOSE (+) = YELLOW SLANT; (-) RED SLANT

GLUCOSE (+) YELLOW BUTT; (-) RED BUTT

H2S (+) BLACK BUTT

Question 13

Carbyhydrate Fermention

Answer 13

(+) CARB FERMENTION = YELLOW

(-) CARB FERMENTION = PURPLE

GAS (+) = GAS IN TUBE

Question 14

What is this?

Answer 14

Gram Positive Cocci

Question 15

What is this?

Answer 15

Endospore Stain

ENDOSPORES = GREEN

VEGETATIVE CELLS = REDISH