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Micro Lab Exam 1 > Differential Tests > Flashcards

Differential Tests Flashcards

1
Q

Oxidative/Fermentive Test – Medium

A

O/F Media

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2
Q

Oxidative/Fermentive Test – Reagent

A

glucose

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3
Q

Oxidative/Fermentive Test – Enzymes

A

glucose metabolic enzymes

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4
Q

Oxidative/Fermentive Test – Indicator

A

brom thymol blue

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5
Q

Oxidative/Fermentive Test – Positive or Negative Result

A

(+) color change yellow

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6
Q

Purple Broth – Media

A

purple broth base plus carbs

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7
Q

Purple Broth – Reagent

A

glucose, lactose, or sucrose

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8
Q

Purple Broth – Product

A

acid and/or gas

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9
Q

Purple Broth – Indicator

A

brom cresol purple and durham tube

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10
Q

Purple Broth – Positive/Negative Result

A

(+) yellow + bubbles

(-) no color change, no bubbles

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11
Q

Methyl Red/Voges-Proskauer Test – Media

A

MRVP broth

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12
Q

Methyl Red/Voges-Proskauer Test – Reagent

A

glucose

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13
Q

Methyl Red/Voges-Proskauer Test – Enzyme

A

glucose metabolic enzymes

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14
Q

Methyl Red/Voges-Proskauer Test – Positive/Negative Result

A

VP+ red in 60min

MR+ red immediately

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15
Q

Nitrate Reductase Test – Media

A

nitrate broth

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16
Q

Nitrate Reductase Test – Reagent

A

potassium nitrate

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17
Q

Nitrate Reductase Test – Products

A

nitrite and nitrogen gas

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18
Q

Nitrate Reductase Test – Enzyme

A

nitrate reductase

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19
Q

Nitrate Reductase Test – Positive/Negative Result

A

(+) gas and blue color change

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20
Q

Citrate Test – Media

A

Simmon’s Citrate Agar

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21
Q

Citrate Test – Reagent

A

sodium citrate

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22
Q

Citrate Test – Enzymes

A

citrate permease and citrase

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23
Q

Citrate Test – Indicator

A

brom thymol blue

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24
Q

Citrate Test – Positive/Negative Result

A

(+) blue and growth

(-) green

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25
Q

Catalase Test - Products

A

gas

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26
Q

Catalase Test – Enzymes

A

catalase

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27
Q

Catalase Test – Positive/Negative Result

A

(+) bubbles

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28
Q

Oxidase Test – Positive/Negative Result

A

(+) bubbles

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29
Q

Phenylalanine Deaminase Test – Media

A

phenylalanine agar slant

30
Q

Phenylalanine Deaminase Test – Reagents

A

phenylalanine

31
Q

Phenylalanine Deaminase Test – Product

A

phenyl pyruvic acid

32
Q

Phenylalanine Deaminase Test – Enzyme

A

phenylalanine deaminase

33
Q

Phenylalanine Deaminase Test – Indicator

A

ferric chloride

34
Q

Phenylalanine Deaminase Test – PositiveNegative Result

35
Q

Starch Hydrolysis Test – Media

A

starch agar

36
Q

Starch Hydrolysis Test – Reagent

37
Q

Starch Hydrolysis Test – Enzyme

38
Q

Starch Hydrolysis Test – Indicator

39
Q

Starch Hydrolysis Test – Positive/Negative Result

A

(+) clear zone around growth

40
Q

Urea Hydrolysis Test – Media

A

urease test broth

41
Q

Urea Hydrolysis Test – Reagent

42
Q

Urea Hydrolysis Test – Product

43
Q

Urea Hydrolysis Test – Enzyme

44
Q

Urea Hydrolysis Test – Indicator

A

phenol red

45
Q

Urea Hydrolysis Test – Positive/Negative Result

A

(+) red

(-) yellow

46
Q

Gelatin Hydrolysis Test – Media

A

gelatin agar plate

47
Q

Gelatin Hydrolysis Test – Reagent

48
Q

Gelatin Hydrolysis Test – Enzyme

A

gelatinase

49
Q

Gelatin Hydrolysis Test – Positive/Negative Result

A

(+) clear zone around growth

50
Q

Sulfur-Indole-Motility Test – Media

51
Q

Sulfur-Indole-Motility Test – Reagents

A

tryptophan

52
Q

Sulfur-Indole-Motility Test – Product

53
Q

Sulfur-Indole-Motility Test – Indicator

A

Kovac’s Reagent

54
Q

Sulfur-Indole-Motility Test – Positive/Negative Result

A

(+) cherry red

55
Q

Sulfur-Indole-Motility Test – Enzyme

A

tryptophanase

56
Q

Kligler’s Iron Agar – Media

A

Kligler’s Iron Agar Slant

57
Q

Kligler’s Iron Agar – Reagents

A

glucose and lactose

58
Q

Kligler’s Iron Agar – Product

59
Q

Kligler’s Iron Agar – Indicator

A

phenol red

60
Q

Kligler’s Iron Agar – Positive/Negative Result

61
Q

Thiosulfate Reductase Test – Media

A

Kligler’s Iron Agar slant

62
Q

Thiosulfate Reductase Test – Reagent

A

sodium thiosulfate

63
Q

Thiosulfate Reductase Test – Product

64
Q

Thiosulfate Reductase Test – Enzyme

A

thiosulfate reductase

65
Q

Thiosulfate Reductase Test – Indicator

A

ferrous sulfate

66
Q

Thiosulfate Reductase Test – Positive/Negative Result

67
Q

Pseudosel Agar Test – Media

A

Pseudosel Agar slant

68
Q

Pseudosel Test – Positive/Negative Result

A

(+) Neon green/yellow

69
Q

Pseudosel Test – what compounds make the green and yellow colors?

A 
green = pyocyanin
yellow = flourescine
70
Q

Inoculating from broth = ____

71
Q

Inoculating from slant = ___

Micro Lab Exam 1 (2 decks)

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