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Micro Lab Exam 1 > Differential Tests > Flashcards

Differential Tests Flashcards

1
Q

Oxidative/Fermentive Test – Medium

A

O/F Media

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2
Q

Oxidative/Fermentive Test – Reagent

A

glucose

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3
Q

Oxidative/Fermentive Test – Enzymes

A

glucose metabolic enzymes

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4
Q

Oxidative/Fermentive Test – Indicator

A

brom thymol blue

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5
Q

Oxidative/Fermentive Test – Positive or Negative Result

A

(+) color change yellow

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6
Q

Purple Broth – Media

A

purple broth base plus carbs

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7
Q

Purple Broth – Reagent

A

glucose, lactose, or sucrose

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8
Q

Purple Broth – Product

A

acid and/or gas

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9
Q

Purple Broth – Indicator

A

brom cresol purple and durham tube

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10
Q

Purple Broth – Positive/Negative Result

A

(+) yellow + bubbles

(-) no color change, no bubbles

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11
Q

Methyl Red/Voges-Proskauer Test – Media

A

MRVP broth

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12
Q

Methyl Red/Voges-Proskauer Test – Reagent

A

glucose

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13
Q

Methyl Red/Voges-Proskauer Test – Enzyme

A

glucose metabolic enzymes

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14
Q

Methyl Red/Voges-Proskauer Test – Positive/Negative Result

A

VP+ red in 60min

MR+ red immediately

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15
Q

Nitrate Reductase Test – Media

A

nitrate broth

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16
Q

Nitrate Reductase Test – Reagent

A

potassium nitrate

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17
Q

Nitrate Reductase Test – Products

A

nitrite and nitrogen gas

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18
Q

Nitrate Reductase Test – Enzyme

A

nitrate reductase

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19
Q

Nitrate Reductase Test – Positive/Negative Result

A

(+) gas and blue color change

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20
Q

Citrate Test – Media

A

Simmon’s Citrate Agar

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21
Q

Citrate Test – Reagent

A

sodium citrate

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22
Q

Citrate Test – Enzymes

A

citrate permease and citrase

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23
Q

Citrate Test – Indicator

A

brom thymol blue

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24
Q

Citrate Test – Positive/Negative Result

A

(+) blue and growth

(-) green

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25
Catalase Test - Products
gas
26
Catalase Test -- Enzymes
catalase
27
Catalase Test -- Positive/Negative Result
(+) bubbles
28
Oxidase Test -- Positive/Negative Result
(+) bubbles
29
Phenylalanine Deaminase Test -- Media
phenylalanine agar slant
30
Phenylalanine Deaminase Test -- Reagents
phenylalanine
31
Phenylalanine Deaminase Test -- Product
phenyl pyruvic acid
32
Phenylalanine Deaminase Test -- Enzyme
phenylalanine deaminase
33
Phenylalanine Deaminase Test -- Indicator
ferric chloride
34
Phenylalanine Deaminase Test -- PositiveNegative Result
(+) green
35
Starch Hydrolysis Test -- Media
starch agar
36
Starch Hydrolysis Test -- Reagent
starch
37
Starch Hydrolysis Test -- Enzyme
amylase
38
Starch Hydrolysis Test -- Indicator
iodine
39
Starch Hydrolysis Test -- Positive/Negative Result
(+) clear zone around growth
40
Urea Hydrolysis Test -- Media
urease test broth
41
Urea Hydrolysis Test -- Reagent
urea
42
Urea Hydrolysis Test -- Product
NH3
43
Urea Hydrolysis Test -- Enzyme
urease
44
Urea Hydrolysis Test -- Indicator
phenol red
45
Urea Hydrolysis Test -- Positive/Negative Result
(+) red | (-) yellow
46
Gelatin Hydrolysis Test -- Media
gelatin agar plate
47
Gelatin Hydrolysis Test -- Reagent
gelatin
48
Gelatin Hydrolysis Test -- Enzyme
gelatinase
49
Gelatin Hydrolysis Test -- Positive/Negative Result
(+) clear zone around growth
50
Sulfur-Indole-Motility Test -- Media
SIM media
51
Sulfur-Indole-Motility Test -- Reagents
tryptophan
52
Sulfur-Indole-Motility Test -- Product
indole
53
Sulfur-Indole-Motility Test -- Indicator
Kovac's Reagent
54
Sulfur-Indole-Motility Test -- Positive/Negative Result
(+) cherry red
55
Sulfur-Indole-Motility Test -- Enzyme
tryptophanase
56
Kligler's Iron Agar -- Media
Kligler's Iron Agar Slant
57
Kligler's Iron Agar -- Reagents
glucose and lactose
58
Kligler's Iron Agar -- Product
acid
59
Kligler's Iron Agar -- Indicator
phenol red
60
Kligler's Iron Agar -- Positive/Negative Result
(+) yello
61
Thiosulfate Reductase Test -- Media
Kligler's Iron Agar slant
62
Thiosulfate Reductase Test -- Reagent
sodium thiosulfate
63
Thiosulfate Reductase Test -- Product
H2S
64
Thiosulfate Reductase Test -- Enzyme
thiosulfate reductase
65
Thiosulfate Reductase Test -- Indicator
ferrous sulfate
66
Thiosulfate Reductase Test -- Positive/Negative Result
(+) black
67
Pseudosel Agar Test -- Media
Pseudosel Agar slant
68
Pseudosel Test -- Positive/Negative Result
(+) Neon green/yellow
69
Pseudosel Test -- what compounds make the green and yellow colors?
``` green = pyocyanin yellow = flourescine ```
70
Inoculating from broth = ____
loop
71
Inoculating from slant = ___
needle

Micro Lab Exam 1 (2 decks)

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