Differential Tests Flashcards
Oxidative/Fermentive Test – Medium
O/F Media
Oxidative/Fermentive Test – Reagent
glucose
Oxidative/Fermentive Test – Enzymes
glucose metabolic enzymes
Oxidative/Fermentive Test – Indicator
brom thymol blue
Oxidative/Fermentive Test – Positive or Negative Result
(+) color change yellow
Purple Broth – Media
purple broth base plus carbs
Purple Broth – Reagent
glucose, lactose, or sucrose
Purple Broth – Product
acid and/or gas
Purple Broth – Indicator
brom cresol purple and durham tube
Purple Broth – Positive/Negative Result
(+) yellow + bubbles
(-) no color change, no bubbles
Methyl Red/Voges-Proskauer Test – Media
MRVP broth
Methyl Red/Voges-Proskauer Test – Reagent
glucose
Methyl Red/Voges-Proskauer Test – Enzyme
glucose metabolic enzymes
Methyl Red/Voges-Proskauer Test – Positive/Negative Result
VP+ red in 60min
MR+ red immediately
Nitrate Reductase Test – Media
nitrate broth
Nitrate Reductase Test – Reagent
potassium nitrate
Nitrate Reductase Test – Products
nitrite and nitrogen gas
Nitrate Reductase Test – Enzyme
nitrate reductase
Nitrate Reductase Test – Positive/Negative Result
(+) gas and blue color change
Citrate Test – Media
Simmon’s Citrate Agar
Citrate Test – Reagent
sodium citrate
Citrate Test – Enzymes
citrate permease and citrase
Citrate Test – Indicator
brom thymol blue
Citrate Test – Positive/Negative Result
(+) blue and growth
(-) green
Catalase Test - Products
gas
Catalase Test – Enzymes
catalase
Catalase Test – Positive/Negative Result
(+) bubbles
Oxidase Test – Positive/Negative Result
(+) bubbles