Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Virology > Picornaviruses > Flashcards

Picornaviruses Flashcards

1
Q

Pico rna virus

A

Small RNA virus

Poliovirus, Insensitivity to ether, Coxsackievirus, Orphanvirus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Genome

A

(+)ssRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Structure?

A

Naked icosahedral capsid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

VAP

A

The Canyon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Capsid

A

Made up of VP1-4 with VP4 being internal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

VPg

A

Binds to 5’ end of the genome and acts as a primer for transcription

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Why is expression of the genome infectious?

A

No viral enzymes are packaged, (+)ssRNA so host can translate itself

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Attachment receptor

A

CD155

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Lifecycle

A
  1. Attachment - the Canyon binds CD155
  2. Penetration - Endocytosis which triggers conformational change of capsid
  3. Uncoating/entry - RNA extruded into cytoplasm
  4. Gene expression - primary translation followed by proteolytic processing of polyprotein
  5. Genome replication - (+)ssRNA and replication proteins form double membrane replication organelle. dsRNA intermediate is formed which acts as a template for (+)ssRNA
  6. Assembly
  7. Maturation
  8. Release - lytic or nonlytic
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gene expression

A

Single large polyprotein
VPg is 5’ primer for RNAP (3D)
5’ UTR contains a RNA structure (IRES) for initiation of translation
3’ UTR translation terminated with polyA tail

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

IRES

A

5’ UTR RNA structure used for initiation of translation by recruiting translation initiation factors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Polyprotein protease processing

A

Viral proteases cleave at recognition sites to produce multiple replication and capsid proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Transmission

A

Oral fecal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Primary site of replication

A

Intestinal epithelial cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Secondary replication site

A

CNS or PNS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Polio Pathogenesis

A

Polio walks its endocytic vesicle along microtubules using host dynein to get close to nerve cell body

Uncoats near nerve cell body

Replication causes functional cell death by protein synthesis shut down or lysis

17
Q

Inactivated Polio Virus (IPV) (Salk)

A

Formalin-inactivated virus
No risk of polio
Protects CNS cells NOT intestinal epithelium
DOES NOT STOP SPREAD

18
Q

Oral Polio Vaccine (OPV) (Sabin)

A

3 attenuated serotypes BUT 2 mutations are sufficient for reversion to WT and polio
Cheap
Mucosal immunity
STOPS SPREAD and even spreads contact immunity by she’d of attenuated virus

19
Q

Pros of Sabin OPV

A

Cheap
Mucosal immunity
Stops spread
Contact immunity (also sorta con if immunicompromised)

20
Q

Cons of Sabin OPV

A

Contact immunity could cause polio if immunicompromised
Reversion to WT sometimes

Virology (18 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions