Phytoplankton and sampling Flashcards

1
Q

What is the most abundant species of phytoplankton in the oceans?

A

Diatoms are one of the most abundant groups of phytoplankton in oceans, are more commonly found in temperate latitudes.

In the Atlantic they’re very dominant and form long chains to help suspend themselves in the water column in the photic zone for photosynthesis.

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2
Q

Which shapes can diatoms form?

A

Pennate = rods,bilateral symmetry (can be benthic) Only pennate diatoms are capable of locomotion – wave like motion on surface – only possible when diatom is in contact with another surface – generally restricted to shallow water sediments & surfaces larger plants/animals

Centric = petri dish shape, radial symmetry, Centric diatoms – tend to always be planktonic in the water column.

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3
Q

Describe the anatomy of a diatom

A

Diatoms all have external skeletons made of silica called the frustule which is made up of two halves.

1st half, the epitheca is larger than the other forming a ‘lid’.

2nd half, the hypotheca is smalla forming the ‘box’ part.

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4
Q

Describe how diatoms reproduce asexually

A

Parent cell divides to produce 2 daughter cells (simple asexual division. The cell forms 2 nuclei, 2 halves of frustule split. Each daughter cell grown new inner half.

Method can yield large number in short period – take advantage of improved growth conditions
This method of cell division recycles the old frustules – results slight decrease in ave. cell size with each division

Diatom forms another frustule inside the original frustrule. The epitheca grows a new hypotheca to regenerate new cell. The hypotheca becomes the new epitheca on the new cell. So cells get smaller through reproduction.

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5
Q

How often can diatoms divide under optimum light and nutrient conditions?

A

Every 12-24 hours making number swell and producing a bloom.

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6
Q

Describe how diatoms reproduce sexually

A

Due to the reduction in size during asexual reproduction, once daughter cells reach their critical minimum size sexual reproduction will take place.

Cells loose the frustule and half their genetic material.

They will meet another cell in the same condition and fuse to make a zygote which grows into an auxospore.

The frustule returns to it’s normal size so asexual reproduction begins again.

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7
Q

Describe a Coccolithophore

A

Unicellular, nanoplankton (2-20 nanometres)

Made up of round circles are coccolith plate made up of calcium carbonate. The plates come together to form a Coccolithophore. Effected by ocean acidification because they’re made up of primarily calcium carbonate. Important in the production of chalk.

They’re important in tropical and subtropical waters

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8
Q

Describe a Silicoflagellate

A

They have internal ornate silica skeletons with one or two flagella which help with mobility and many small chloroplasts.

Their contribution to primary production is smaller than that of diatoms and coccolithophores but is still significant.

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9
Q

Describe a Dinoflagellate

A

Usually solitary with two flagella and between 25-1000 nanometres.

They dominate tropical regions and autumn temperate blooms.

Most dinoflagellates possess a thick cellulose wall called a theca. Without a theca dinoflagellates are considered as ‘naked’.

These phytoplankton don’t form chains or aggregations.

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10
Q

What are the two types dinoflagellates and their properties

A

Desmophyceae
-2 longlitudunal halves with flagella both on the anterior.

Dinophyceae
-Made up of anterior and posteria halves with 1 flagella girdle and 1 flagella posterior

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11
Q

How do dinoflagellates reproduce asexually?

A

Longitudinal cell division – each new daughter cell retains part of old cell wall & rebuilds missing part after cell division

Longitudinal cell division – each new daughter cell retains part of old cell wall & rebuilds missing part after cell division

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12
Q

How do dinoflagellates reproduce sexually?

A

Sexual reproduction will only take place under very harsh conditions such as; not enough light, nutrients or too much turbidity.

New cells are dormant thick walled cysts on the seabed.

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13
Q

Can dinoflagellates cause a harmful toxic bloom?

A

Yes, their biolumin (lucifrin protein) is oxidised which is a toxin and causes harmful bloom which is of concern for the public and the environment.

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14
Q

What is the most common method for sampling plankton?

A

A large cone shaped net.

This can be used vertically, obliquely and horizontally.

It filters larger volumes so is useful for less abundant species.

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15
Q

Who invented the continuous plankton recorder and how does it work?

A

Sir Alister Hardy invented it in 1926.

The filtering silk moves around the columns continuously to allow looking at the spatial distribution of plankton. Towed off the back of the ships of opportunity. E.g the ferry that moves from Plymouth to france continuously. Like tankers for example. This has been happening since the 1930’s

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16
Q

What has the continuous plankton recorder shown?

A

It has generated the most understanding of the impact of climate change on lower trophic levels.

It has allowed for samples to be collected over large spatial scales. It is the basis for the largest plankton survey in the world.

17
Q

What did the continuous plankton recorder show in the north-east Atlantic in terms of bio-geographical range extensions.

A

CPR data shows progressive increase in sub-tropical species in temperate latitudes.

  • sub-tropical cladoceran Penila avirostris has had a significant increase in abunance.
  • Juveniles of stalked barnacle (Lepas species) now found near Iceland.
  • Dinoflagellate Ceratium trichoceros previously limited to southern UK now found in Scottish waters and N. North Sea.
  • Copepods in North Sea switching from Calanus finmarchicus to C. helgolandicus.
18
Q

Why has their been a switch in species of copepods?

A

Warm water loving species of copepod and cold water loving copepod. So because the water was warming the copepods started to change their distribution because of climate change in the north sea. Could change their depth due to higher radiation levels

19
Q

What kind of sampling takes place at the Western Channel Observatory

A

Vertical net hauls, benthic trawls.

20
Q

What methods are used to determine spatial distribution of phytoplankton?

A

Direct microscope counts for abundance.

Fluorometry to understand cell volume using image analysis.

Remote sensing

Composite images are used to look at spatial and seasonal patterns.

21
Q

What are the global trends for phytoplankton?

A

Phytoplankton are most abundant in coastal regions.

  • Nutrient inputs to coastal zone from riverine sources.
  • Upwelling in some areas
  • Mixing in relatively shallow waters

Phytoplankton tends to decrease with distance from land.

  • due to lack of nutrient inputs
  • little mixing between gyres and coastal waters
  • exception of equatorial upwelling
22
Q

What problems can arise from eutophication? (20 mile long blooms)

A

Physical effects of dense mats of algae blocking intake pipes and filtering systems. Can block light to water column underneath.

23
Q

What happened because of a harmful algal bloom due to the neurotoxin saxitoxin in dinoflagellates?

A

The neurotoxin passed up through the food chain from filter feeders to fish, mammals and humans causing paralytic shellfish poisoning.

This causes numbness, nauseam paralysis of breathing which can lead to cardiac arrest and death.

There was no antidote and the toxin couldn’t be killed by cooking.

This lead to frequent bans on commercial and recreational shellfish harvesting.

The first case was in borneo in 1976 where 200 people fell ill and 7 people died.

24
Q

What methods have been experimented with to try identify toxic blooms around fisheries to reduce public health issues?

A

Using the colour on satellite imagery

25
Q

How does the CDT rosette work and sample phytoplankton?

A

CDT rosette (top right image) conductivity depth and temp meters which connected to the rig. Each of the big bottles is 20 litres. Top and the bottom are on mechanisms which remain open when put into the water so water moves through the tubes until the sampling depth is reached. Top and bottom are closed to collect desired water.

26
Q

What did the CDT rosette used in the expedition from Falmouth UK to Chile reveal?

A

1st evidence of methylotrophic bacteria in oligotrophic gyres.

This is bacteria that uses methanal to grow