Phytoplankton Flashcards

1
Q

the first phytoplankton

A

coccoid cyanobacteria

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2
Q

2nd phytoplankton

A

evolved from the engulfing of a photosynthetic prokaryote by an aerobic eukaryote
From this, two groups evolved- the Green Algae and the Red Algae

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3
Q

Three groups arose from the primary endosymbiosis…what came from secondary endosymbiosis

A

Euglenoids by ingestion of a green alga by a protozoan

Heterokonts (brown algae, chrysophytes and diatoms)

Cryptomonads
Haptophytes (Prymnesiophytes)

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4
Q

Apicomplexans

A

These are protozoan parasites such as malaria

The Chlorarachniophytes are Amoebas that have green chloroplasts

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5
Q

Cyanobacterial Characteristics

A

Prokaryotes: no membrane bound organelles (no nucleus etc.)
Circular DNA, no histone protein
Pigments: Chl a, Beta Carotine, Zeaxanthin, phycobiliproteins (Prochlorophytes have a divinyl Chl a & Chl b)
Rubisco (C fixation) in carboxysomes
Lack flagella
Cyanophycin: aspartic acid & arginine (N storage)

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6
Q

Thylacoids

A

flattened membrane sacks with photosynthetic pigments present

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7
Q

Carboxysomes

A

sites of C fixation

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8
Q

Cyanophycin

A

granules are for N storage

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9
Q

Polyphosphate

A

granules are site of P storage

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10
Q

Phycobiliproteins

A

are accessory pigments (absorb light energy at wavelengths where chl a doesn’t absorb).

Water soluble pigments with a structure like bile

Together form a phycobilisome near to PS I & PSII pigments
Very high efficiency of energy transfer to Chl a

Open Chain tetrapyrroles. Phycoerythrin (PE), phycocyanin (PC), allophycocyanin

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11
Q

Other accessory pigments

A

Carotenoids

Beta carotene

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12
Q

Carotenes

A

consist of long chain hydrocarbons & absorb shorter wavelengths (~400-500 nm). They don’t contain oxygen

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13
Q

Xanthophylls

A

like Carotenes but they contain oxygen

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14
Q

Five Orders of Cyanobacteria

A

Chroococcales, Pleurocapsales, Oscillatoriales, Nostocales, Stigonematales:

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15
Q

Chroococcales

A

Unicellular or in aggregates

Cyanobacteria

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16
Q

Pleurocapsales

A

unicellular to complex aggregates

Cyanobacteria

Can bore into calcareous rock and grow endolithically. Acid producing bacteria grow with them and do boring. Divide by multiple (1 to 4)or binary fission (1to 2) or baeocytes (1 to many) in mother cell.
Only few marine species

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17
Q

Oscillatoriales

A

filamentous (undifferentiated cells), no heterocysts

Cyanobacteria

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18
Q

Nostocales

A

filamentous & with heterocysts

Cyanobacteria

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19
Q

Stigonematales

A

filamentous & true branching, with heterocysts

Cyanobacteria

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20
Q

Planktonic cyanobacterial N2 fixers

A

Richelia intracellularis in some diatoms-lives as symbiont
Trichodesmium (5 species) all colonies
Katagnymene- single trichomes (filaments) with mucus sheath
Unicellular cyanos ~ 3-5 um in dia (Crocosphaera & Cyanothece). Fix N2 at night

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21
Q

Stromatolites

A

cyanobacterial mats with layering of sand

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22
Q

——- is O2 sensitive

A

Nitrogenase

Heterocysts protect from O2

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23
Q

How is phaeophytin different from chlorophyll a?

A

is that chlorophyll is chlorophyll (green pigment) while pheophytin is (biochemistry) a chlorophyll from which the central magnesium atom has been removed.

24
Q

Major source of iron for ocean?

25
Pelagophyceae
Unicellular, brown tides, Grow so dense that they shade seagrasses, thus killing them.
26
Prasinophytes (Micromonadophyceae)
Primitive green flagellates with Chl a & b 4-8 flagella Covered with scales Coastal waters & tidepools
27
what is Convoluta?
flatworm lives in sand and has algal symbiont Prasinophyte in tissue Forms green patches on sand and as tide comes in disappears into sediments Worms rely on photosynthate Older worms eat the algae from tail to head then die and releases eggs with alga
28
Raphidophytes
Two flagella, chl a & c & fucoxanthan Vertically migrates 10-15 m daily Has cyst stage Toxic to fish, especially caged ones. In ’72 Chatonella killed $500 mil of Yellow tail fish in Japan
29
Chrysophyceae
“Golden Algae” Very diverse group, related to Prymnesiophytes but with no haptonema Pigments: Chl a & c, B Carotene, Fucoxanthan, Diadinoxanthan, Violaxanthan Storage: Chrysolaminarin Most freshwater, found in tide pools Usually 2 unequal flagella or just one statospore (of silicon) for hard times
30
Cryptomonads
``` Chl a & c, Phycocyanin & phycoerythrin 2 equal or unequal flagella Storage: Starch Thylacoids in pairs Have a gullet lined with trichocysts Very abundant in brackish water ```
31
Dinoflagellates
Basically a protozoan that has acquired chloroplasts Most are motile & unicellular ~ 7 μm-2 mm in size Some are colorless and can be phagotrophic or (heterotrophs) Some totally autotrophic Some are mixotrophs (both auto and heterotrophic) and can both photosynthesize and consume other phytoplankton Some are symbionts (in corals & other organisms) Some are parasites
32
dinoflagellates pigments?
Chl a & c, B Carotene, Peridinin (signature pigment), rarely fucoxanthan & diatoxanthan About 2000 species (90% marine) Thylacoids in stacks of 3 Chloroplasts enclosed by double unit membrane
33
Three Orders of Dinoflagellates
Prorocentrales (2 cellulose plates) Dinophysidales (2 large cellulose plates & 12 small plates) Peridiniales (many plates but some are without plates “naked”)
34
When do we call dinoflagellates “naked”?
if they do not have any cellulose plates
35
what do dinoflagellates have to sting prey?
trichocysts
36
complex life cycle of dinoflagellates
Swimmers are haploid vegetative cells When conditions become unfavorable, gametes are formed and a hystrichospheroid cyst (diploid) forms, with resistant sporopollinin cell wall, and falls to sediments When conditions favorable, it excysts, undergoes meiosis and new vegetative cells formed Pellicle cysts can also be formed for short term survival.
37
Why do dinoflagellates bioluminesce?
Reduces predation, Flashing dinos when copepods try to eat them attract a predator
38
What are plates made of in dinoflagellates?
cellulose (armor)
39
When bioluminescence brightest and what is the value of it?
new moon, reduces predation
40
Why do dinoflagellates vertically migrate?
- food - -Many dinoflagellates vertically migrate into the nitricline at night to take up NO3 and to upper euphotic zone in morning to photosynthesize in the daytime. Shown is dino vertical migration in mesocosm
41
Margalef’s Mandala
and provides a route map through the variations of phytoplankton composition in time and space, and the causes of these variations.
42
Diatoms (Bacillariophyceae)
Consist of ~ 12,000 species Plankton or Periphyton (attached) 4-5 μm- >1 mm Pigments: Chl a & c, B carotene, Fucoxanthan, Diadinoxanthin, Diatoxanthin Consist of two orders, Centrales & Pennales Chloroplast has thylacoids in groups of three Storage product is chrysolaminarin (B 1-3 linked glucose units) and lipids (5-15% dry wt young-40-50% when old)
43
Diatom Frustule made of
Silicon & Silicate & Silica
44
Diatom Frustule
the hard and porous cell wall or external layer of diatoms Nutrients are exchanged through holes in the frustule. Holes are called punctae in pennates, and in centrics there are sieve like structures (areolae) Outer buffer zone of frustule has a structural carbohydrate layer plus lipids and an amino acid template with a high serine content Pennates can move if attached to something. The raphe is the long slit down the middle of the frustule and is involved in movement. Centrics have no raphe.
45
Two orders of diatoms
Centrales & Pennales
46
tell me about the buoancy of diatoms...
frustule is heavy, regulate the ionic content of the cell sap in vacuole. They replace heavy ions such as Ca & Mg with lighter ions such as Na or K. This replacement actually makes them buoyant. Shape of the cell influences sinking velocity and elaboration of cell shape increases surface area ratio and this increases viscous drag as it sinks
47
MacDonald Pfitzer Rule
When a diatom divides, each of the original halves become the epitheca. Thus half of the cells become progressively smaller
48
how do diatoms get back to thier OG size?
original large size by production of auxospores. Auxospores produced after sexual recombination
49
what are diatom blooms dependant on?
a supply of silicic acid, hence typically occur in coastal areas.
50
where are diatoms going to be the most prevalent?
where there is lots of silicic acid... coastal areas which receive riverine input Upwelling regions also have high diatom abundance and silicic acid concentrations are higher in deep water which upwells Antarctic waters of the Southern Ocean have high concentrations of silicic acid and diatoms Sedemented as opal (a biogenic mineral)
51
what are Resting spores (diatoms)?
When a bloom is over and nutrients are exhausted, diatoms form resistant resting spores. Spores allow a short term (months) survival in sediments
52
How genetically diverse are diatoms
In 2 years Jane Gallager (J. Phycol 1980 vol 16:464) isolated 457 clones of Skeletonema costatum. Clones isolated in winter were genetically distinct from summer ones They were as different as two different species but morphologically they all were identical. No single isolate is representative of all populations
53
When and why do diatoms have sex?
in order to avoid becoming smaller and smaller (MacDonals Rule) they need to reproduce sextually
54
What concentration of silicic acid starts to become limiting? (diatoms)
Diatoms dominate when silicic acid concentrations are above 2 uM they have a hard time at lower concentrations
55
How diatoms retard copepod egg production?
The diatoms synthesize aldehyde molecules which arrest mitosis in eggs.
56
What are Silaffins
Silaffins are involved in the formation of the cell walls of diatoms
57
Punctae & aerolae (diatoms)
Aerolae-- A general term for a round or oval pore in the silica wall of a diatom Punctae -- the plural form