Physiochemistry of Haemagglutination Reactions & Antibody Detection Techniques

Question 1

specificity is dependant on

Answer 1

structure and charge

Question 2

is held together by a combination of weak non-covalent forces
what are these forces

Answer 2

electrostatic forces (Ionic Bonding)
hydrogen bonds
hydrophobic bonds
van der Waals forces

Question 3

the association constant (Ka) measures

Answer 3

the affinity of the interaction between antigen and antibody

Question 4

detection of Ag:Ab reactions in immunological assays include:

Answer 4

agglutination
precipitation
haemolysis
radioimmunoassay (RIA)
Immunofluorescence
Immuno-magnetic labelling
enzyme linked immunosorbant assay (ELISA)
solid phase adherence

Question 5

haemolysis may also indicate presence

Answer 5

of an Ag:Ab reaction

Question 6

haemagglutination (red cell agglutination) is the end-point for

Answer 6

most techniques

Question 7

Equivalence Zone: Ab:Ag ratio is optimum for

Answer 7

maximum visible agglutination reaction

Question 8

primary stage: Sensitisation (Coating)

Answer 8

antibody binds to antigen on the red cell surface

Question 9

secondary stage: Haemagglutination

Answer 9

antibody bound on one red blood cell (rbc) interacts with antigen on a second cell
intermolecular bridge formation creates a 3D latticework of visible red cell aggregates