PHYSICAL METHODS OF STERILIZATION

Question 1

steam under pressure

Answer 1

autoclave

Question 2

• 121 degree Celsius 15 psi for 15-30 mins
• 132 degree Celsius 15 psi for 30-60 mins

Answer 2

Autoclave

Question 3

100 degree Celsius for 30 mins in 3 consecutive days

Answer 3

Tyndallization/Fractional Sterilization

Question 4

70-80 degree Celsius 2 hrs for 3 consecutive days

Answer 4

Inspissation

Question 5

for heat sensitive media like LJ media

Answer 5

Inspissation

Question 6

Moist Heat sterilization Methods

Answer 6

• Autoclave
• Tyndallization/Fractional Sterilization
• Inspissation

Question 7

160-170 degree Celsius for 1.5-3 hrs

Answer 7

Oven

Question 8

Dry Heat Method of Sterilization

Answer 8

Oven

Question 9

temp for incineration

Answer 9

870-980 degree Celsius

Question 10

not done in the PH due to clean air act

Answer 10

incineration

Question 11

for heat sensitive materials such as antibiotic solutions, toxic chemicals, radioisotopes, vaccines, carbohydrates

Answer 11

Membrane Filtration

Question 12

membrane filtration pore size

Answer 12

usual: 0.45-0.80
parenteral solutions: 0.22 um
small viruses: 0.1 um

Question 13

pore size of HEPA filter

Answer 13

0.3 um

Question 14

HEPA Filter

Answer 14

High Efficiency Particulate Air

Question 15

composed of short wavelength, high energy gamma rays

Answer 15

ionizing/gamma radiation

Question 16

used in sterilizing disposables such as plastic syringe, vacutainer blood tubes, catheters, gloves

Answer 16

ionizing/gamma radiation