GRAM (-) MEDIUM Flashcards by Queen Alcaraz

Q

Carbohydrate present in EMB (Levine Agar)

A

Lactose and Sucrose

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Q

EMB Indicator and inhibitory for Gram +

A

Eosin Y and Methylene Blue

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Q

Lactose Fermenter (LF) in EMB

A

Dark Purple to Black

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Q

Non Lactose Fermenter (NLF) in EMB

A

Colorless or Translucent

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Q

E. coli in EMB

A

green metallic sheen

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Q

E. aerogenes in EMB

A

fish eye colony

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Q

inhibitory for gram + in MAC

A

crystal violet, bile salt

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Q

carbohydrate present in MAC

A

lactose

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Q

MAC indicator

A

neutral red

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Q

LF in MAC

A

pink/red

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Q

NLF in MAC

A

colorless

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Q

detection of E. coli O157:H7

A

MAC Sorbitol

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Q

inhibitory for Gram + in XLD

A

bile salt

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Q

XLD Carbohydrate

A

Lactose, Xylose, Dextrose

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Q

XLD indicator

A

phenol red

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Q

LF in XLD

A

yellow

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Q

NLF in XLD

A

red/colorless

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Q

XLD is selective and differential for

A

Salmonella and Shigella

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Q

inhibitory for Gram + in HEA

A

bile salt, bromithymol blue, acid fuschkn

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Q

HEA carbohydrate

A

Lactose, Salicin, Sucrose

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Q

HEA indicator

A

Bromothymol blue

Q

LF in HEA

A

orange to salmon pink

Q

NLF in HEA

A

blue green/colorless

Q

differentiates salmonella and shigella from other enteric gram negative bacteria

A

HEA

inhibitory for Gram + for DCA

bile salts

Carbohydrate for DCA

Lactose

DCA indicator

neutral red

LF in DCA

red or pink

NLF in DCA

colorless

inhibitory for Gram + SSA

brilliant green, bile salt

carbohydrate in SSA

Lactose

SSA indicator

Neutral red

LF in SSA

red/pink

NLF in SSA

colorless

for Salmonella and Shigella

SSA, DCA

inhibitory for Gram + BSA

brilliant green

carbohydrate in BSA

Lactose

BSA indicator

bismuth sulfite

salmonella (NLF) in BSA

black

for Salmonella

BSA

inhibitory for Gram + TCBS

bile salts

carbohydrate in TCBS

sucrose

indicator of TCBS

bromothymol blue

LF in TCBS

yellow

NLF in TCBS

green (sucrose)

TCBS is used for

Vibrio

indicator for XLD

phenol red

indicator of DCA, SSA, MAC

Neutral red

indicator for HEA, TCBS

bromothymol blue

indicator of EMB

eosin Y and Methylene blue