GRAM (-) MEDIUM Flashcards
Carbohydrate present in EMB (Levine Agar)
Lactose and Sucrose
EMB Indicator and inhibitory for Gram +
Eosin Y and Methylene Blue
Lactose Fermenter (LF) in EMB
Dark Purple to Black
Non Lactose Fermenter (NLF) in EMB
Colorless or Translucent
E. coli in EMB
green metallic sheen
E. aerogenes in EMB
fish eye colony
inhibitory for gram + in MAC
crystal violet, bile salt
carbohydrate present in MAC
lactose
MAC indicator
neutral red
LF in MAC
pink/red
NLF in MAC
colorless
detection of E. coli O157:H7
MAC Sorbitol
inhibitory for Gram + in XLD
bile salt
XLD Carbohydrate
Lactose, Xylose, Dextrose
XLD indicator
phenol red
LF in XLD
yellow
NLF in XLD
red/colorless
XLD is selective and differential for
Salmonella and Shigella
inhibitory for Gram + in HEA
bile salt, bromithymol blue, acid fuschkn
HEA carbohydrate
Lactose, Salicin, Sucrose
HEA indicator
Bromothymol blue
LF in HEA
orange to salmon pink
NLF in HEA
blue green/colorless
differentiates salmonella and shigella from other enteric gram negative bacteria
HEA
inhibitory for Gram + for DCA
bile salts
Carbohydrate for DCA
Lactose
DCA indicator
neutral red
LF in DCA
red or pink
NLF in DCA
colorless
inhibitory for Gram + SSA
brilliant green, bile salt
carbohydrate in SSA
Lactose
SSA indicator
Neutral red
LF in SSA
red/pink
NLF in SSA
colorless
for Salmonella and Shigella
SSA, DCA
inhibitory for Gram + BSA
brilliant green
carbohydrate in BSA
Lactose
BSA indicator
bismuth sulfite
salmonella (NLF) in BSA
black
for Salmonella
BSA
inhibitory for Gram + TCBS
bile salts
carbohydrate in TCBS
sucrose
indicator of TCBS
bromothymol blue
LF in TCBS
yellow
NLF in TCBS
green (sucrose)
TCBS is used for
Vibrio
indicator for XLD
phenol red
indicator of DCA, SSA, MAC
Neutral red
indicator for HEA, TCBS
bromothymol blue
indicator of EMB
eosin Y and Methylene blue