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Batce lab > PHYSICAL METHODS > Flashcards

PHYSICAL METHODS Flashcards

1
Q

for critical sterilization _______ filter must be used

A

0.22 um

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2
Q

Ionizing radiations:

A

Gamma rays or electron beams

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3
Q

filtration of liquids through sintered glass

A

morton

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4
Q

machine used for tyndallization

A

arnold’s sterilizer

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5
Q

outlawed the incinirator

A

RA 8749 Clean air act of 1999

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6
Q

mechanism of action od radiations

A

alkylation of nucleic acids

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7
Q

for disposal of hospital waster

A

incinirator

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8
Q

insipissation temp and duration

A

75-80 c for 2hrs on 3 consecutive days

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9
Q

moist heat principle

A

coagulation of proteins

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10
Q

dehydration

A

dessication

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11
Q

size HEPA filter can remove

A

> 0.3 um

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12
Q

removal of water to stop metabolic action of bacteria

A

dessication

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13
Q

filtration of liquids through unglazed porcelain

A

chamberland-pasteur

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14
Q

is exposure to low temperature bactericidal or bacteriostatic

A

bacteriostatic

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15
Q

most commonly used metthod

A

HEAT

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16
Q

free flowing steam

A

tyndallization

17
Q

for sterilization of inoculating loops, needles and mouth of tubes

A

bunsen burner

18
Q

through plamolysis

A

high osmotic pressure

19
Q

filtration of liquids through asbestos pad

A

seitz

20
Q

boiling temp and duration

A

100 c for 10-15 mins

21
Q

Non ionizing radition:

A

UV rays

22
Q

form of moist heat disenfection that doesent kill pores

A

Boiling

23
Q

using high concentrations of salt/sugar

A

high osmotic pressure

24
Q

principle of dry heat

A

oxidation

25
Q

Autoclave temp, psi, time

A

121 c, 15 PSI, 15 mins

26
Q

methods of pasteurization and their temp and duration

A
  • Batch method (63 c for 30 mins)
  • Flash method (72C for 15 seconds)
  • High tempt Short time (72-140c then 72c for <5 secs)
27
Q

for sterilizing glasswares

A

HOT AIR oven

28
Q

for sterilization of high protein media

A

insipissation

29
Q

eliminates food borne pathogens responsiblle for food spoilage

A

pasteurization

30
Q

filtration of liquids through cellulose ester

A

membrane filters

31
Q

HEPA meaning

A

High Efficiency Particulate Air Filter

32
Q

seperation of bacteria from liquids or air

A

filtration

33
Q

short wavelenght but high energy

A

gamma rays

34
Q

long wavelength but low energy

A

uv rays

35
Q

steriization of disposable materials in the medical industry

A

gamma rays

36
Q

use for disinfection of surfaces

A

UV rays

37
Q

filtration of liquids through diatomaceous earth

A

berkefeld

38
Q

tyndallization temp and duration

A

100c for 30 mins for 3 days

39
Q

temperature requirements for glassware sterilization

A

160-180 c for 1.5-3 hrs

Batce lab (5 decks)

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