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Batce lab > DIFFERENT SPECIMEN COLLECTION > Flashcards

DIFFERENT SPECIMEN COLLECTION Flashcards

1
Q

blood to culture media ration

A

1:10

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2
Q

colony count of _______________ and above is indicative of UTI

A

100,000 CFU/mL

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3
Q

types of UTI

A
  • cystitis
  • urethritis
  • pyelonephritis
  • pyelitis
  • glomerulonephritis
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4
Q

these findings suggests that the sputum is unsatisfactory:

A
  • more than 10 squamous epithelial cells/LPF
  • less than 25 polymorphonuclear leukocytes
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5
Q

recommended volume for CSF

A

> /= 1ml

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6
Q

when do you collect specimen for urine culture

A

early morning

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7
Q

cotton swabs with wooden shaft are not reccomended for:

A

fastidious bacteria (Neisseria)

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8
Q

Aerobic bacteria are collectef from:

A
  • upper respiratory tract
  • nasopharyngeal
  • external ear
  • eye
  • genital tract
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9
Q

what should be done if patient is having trouble expectorating sputum

A

induce by inhaling saline mist

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10
Q

for lesions, wound and abscess, collect the specimen from the

A

advancing margin

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11
Q

bood cultures should be incubated in what temperature

A

37 c

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12
Q

proper volume of sputum to be collected

A

5-10 mL

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14
Q

for deep wound collection

A

aspirate

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15
Q

recommended volume for blood specimen collection for adults and neonates

A
  • 20 ml
  • 1 - 20 ml
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16
Q

best time to collect blood specimen

A

before and after fever spike

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17
Q

as per National Tuberculosis Control Program, for diagnosis of pulmonary tuberculosis, how many specimen should you collect

A

2 (spot and early morning) in seperate days

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18
Q

swabs use for gonococcal urethritis, vaginosis, vaginatis

A

Urogenital swabs

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19
Q

Main anticoagulant for blood culture

A

SPS (0.025-0.030%)

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20
Q

manner of collection for tissue specimens

A

biopsy

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21
Q

when will youu collect specimen?

A

acute phase of the infecction

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22
Q

other specimens for PTB

A
  • transcheal aspirate
  • BAL
  • Bronchial washings
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23
Q

CSF is collected in how many tubes and what ube is for bacteriology

A

3 (2nd for Bacteriology)

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24
Q

collect specimens prior to administration of ___________

A

antibiotics

25
Q

culture used for isolation of group A Streptococcus

A

throat culture

26
Q

body fluids are collected via

A

needle aspiration

27
Q

E.coli and other enterics (Proteus, Enterobacter, Klebsiella), Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, S. saprophyticus are found in what specimen

A

Urine

28
Q

swabs for viral culture:

A
  • cotton
  • dacron
29
Q

collect stool specimen for how many days?

A

3 consecutive days

30
Q

for lesions, wound and abscess what will you identify first?

A

exact anatomic site

31
Q

for diagnosis of meningitis or menigoencephalitis

A

CSF

32
Q

how many blood culture should be done and how many hours apart

A

2-3 culture an hour apart

33
Q

other coagulants used for bood culture:

A
  • heparin
  • sodium amyosulfate
  • sodium citrate
34
Q

Bartlett’s scoring is not applicable for these organisms:

A
  • Mycoplasma
  • Chlamydophila
  • Mycobacteria
  • fungi
  • Legionella
  • Viruses
35
Q

where do you collect Blood specimen?

A

2 - 3 different sites (left and right) within 24 hours

36
Q

H. influenzae, N. meningitidis, S.pneumoniae, S. agalactiae, Listeria can be found in what specimen

A

CSF

37
Q

C. jejuni, Salmonella, Dhigella, E.coli, Vibrio, Y. enterocolitica, L. monocytogenes, C. difficile, MAC are found in what specimen:

A

Stool

38
Q

what will you use for anaerobic bacteria

A

aspirates

39
Q

Gasric tubes used for gastric specimens

A
  • Levine
  • Rehfuss
40
Q

Bartlett’s total score of zero means?

A

lack of inflammatory reaction or contamination with saliva

41
Q

use sterile containers except in:

A

stool collection

42
Q

specimen of choice for the diagnosis of GI pathogens

A

stool

43
Q

swabs for bacterial culture

A
  • dacron
  • calcium alginate
  • rayon
44
Q

manner of collection for CSF

A

Lumbar tap (3rd-4th lumbar vertebra)

45
Q

for diagnosis of lower respiratory tract infections (bacterial pneumonia, PTB)

A

sputum

46
Q

body fluids include:

A
  • abdominal fluid
  • amniotic fluid
  • ascitic fluid
  • bile
  • synovial fluid
  • pleural fluid
  • pericardial fluid
47
Q

can be employed to check the quality or adequacy of the sputum

A

Bartlett’s grading system

48
Q

type of urine collection during cystoscopy

A
  • straight catheterized urine
  • suprapubic aspirates
49
Q

stain for sputum

A

gram

50
Q

types of biopsy

A
  • excisional
  • incisional
51
Q

Media of choice for Blood Culture

A

broth
- BHI
- TSB
- BIphasic Media
- Thioglycollate broth
- commerciall available bottles

Isolator system

52
Q

how many swab sample should you get

A

2

53
Q

for diagnosis of fever of unknown origin, septicemia, bacteremia, typhoid fever, SBE

A

Blood

54
Q

what will you use for aerobic bacteria

A

swab

55
Q

culture used for diagnosis of pertussis, middle ear infections, carrier state of S. aureus, N.meningtidis, H. influenzae

A

Nasopharyngeal cultures

56
Q

what should we avoid as urine specimen:

A
  • random voided urine
  • foley catheters

Batce lab (5 decks)

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