photosynthesis and respiration Flashcards

1
Q

Explain the different requirements of an autotroph and a heterotroph

A

Autotroph – organism that can use an external energy source and

 simple inorganic molecules to make complex organic molecules. The 

 source of carbon is carbon dioxide. The energy source is light, although  

 chemoautotrophs include the nitrifying bacteria. 

 Heterotroph – and organism that needs to take in complex organic 

 molecules ( such as carbohydrates, proteins and lipids) to act as a  

 source of both energy and usable carbon compounds.
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2
Q

Describe the relationship between photosynthesis and respiration in living organisms

A

Photosynthesis provides the organic molecules for respiration which

 oxidises the organic molecules releasing carbon dioxide. The energy  

 flow is in one direction The carbon is recycled.
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3
Q

What range of wavelengths do you think chlorophylls reflect?

A

the green parts of the visible spectrum

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4
Q

what colour light to carotenoids reflect?

A

yellow/orange

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5
Q

Explain how the structure of the grana enables them to carry out their function.

A

Consists of stacks of thylakoid membranes which give a large surface area for ligt absorption

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6
Q

Explain how the structure of the stroma enables chloroplasts to carry out their functions.

A

Fluid filled stroma has enzymes for the Calvin cycle. It surrounds the grana so the products of the light dependent reaction can easily pass into the stroma. DNA has the genetic code to make some of the proteins needed for photosynthesis and there are ribsomes for assembly of these products

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7
Q

Name the two sets of reactions involved in photosynthesis, and state where in the

 chloroplast each occurs.
A

Light –dependent reactions on the lamellae; light independent reaction

 in the stroma
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8
Q

Explain why the very large surface area provided by the grana is important in

 photosynthesis
A

The large surface area allows for more photosynthetic pigments, more

 light absorption and more electron transfer.
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9
Q

List the products of the light –dependent reaction of photosynthesis.

A

ATP, reduced NADP and oxygen

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10
Q

State the role of accessory pigments in a photosystem.

A

They absorb a range of wavelengths of light and pass energy to the

 primary pigment.
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11
Q

Describe the different roles of photosystem I and photosystem II.

A

Photosystem 2 is involved in non-cyclic photophosphorylation and takes part in photoionisation, photosystem 1 is involved in cyclic photophosphorylation and also takes part in photoionisation.

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12
Q

Distinguish between cyclic and non cyclic photophosphorylation

A

In cyclic photophosphorylation electrons return to the pigment from

 which they were emitted. In non-cyclic photophosphorylation electrons  

 pass elsewhere.
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13
Q

Outline the role of water in photosynthesis.

A

It is the source of hydrogen ions to be used in the light –independent stage to reduce GP

to TP. It is an electron donor and replaces the electrons lost by PSII when light excites chlorophyll molecules. It keeps palisade cells turgid, so the full vacuole s push the chloroplasts to the outer edges of the cells where they can more readily trap sunlight and carbon dioxide can diffuse into them

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14
Q

Explain how light causes stomata to open. What is the significance of this?

A

Light is trapped by photosystems (PSI) in the guard cells. AT is made. This actively transports potassium ions into the guard cells. This lowers the water potential of the guard cells and water enters then by osmosis, down a water potential gradient, from the surrounding epidermal cells. The guard cells swell and the tips, where cellulose in the walls is thinner and more flexible, bulge. This pushes guard cells apart from each other, opening the stoma between them.

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15
Q

Suggest why a lack of iron in soil can reduce growth in plants.

A

A lack of iron may mean fewer electron carriers (proteins with haem/iron) in the thylakoid membranes and reduced light –dependent reactions. This could lead to less reduced NADP and fewer molecules of ATP, which would reduce the light –dependent reactions. This in turn reduces yield of amino acids, so fewer proteins for growth or for enzymes needed for the light independent reactions.

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16
Q

State the advantage of using ATP as the universal energy currency.

A

It is efficient because using a single compound makes it easy to control

 and coordinate different energy-requiring processes.
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17
Q

Distinguish between an energy currency molecule and an energy storage molecule.

A

Energy currency : the molecule that is the immediate source of energy

 for reactions in the cell- ATP  

 Energy storage: a large compact, insoluble energy rich  

 molecule – amylose, glycogen or triglyceride.
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18
Q

Explain why ATP is known as the universal energy currency.

A

It releases energy in small manageable amounts for powering chemical reactions in cells

 and it is found in all types of cells.
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19
Q

Explain why living organisms do not have very much NAD and CoA in their cells.

A

Because these molecules are recycled / regenerated.

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20
Q

Alcohol is metabolised in the liver. It is oxidised to ethanol by dehydrogenation, and then

  to ethanoate (acetate). Suggest why people who drink large amounts of alcohol may be  

 deficient in NAD.
A

As the alcohol is metabolised, it undergoes dehydrogenation – removal of hydrogen

 atoms. The hydrogen atoms are combined with NAD, so there is less of it for respiration.
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21
Q

. Explain why NAD is called a nucleic acid/nucleotide derivative.

A

NAD contains ribose sugar (found in RNA), the nitrogenous base adenine and phosphate

 groups. (ATP also contains adenine, ribose and phosphate groups.)
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22
Q

It has been suggested that mitochondria are derived from prokaryotes. What

  features of their structure support this suggestion.
A

Size-most are 1-5µm long – similar to the size of many bacteria.

Contents: They have 70s ribosomes – the same type of ribosomes as are found in bacteria. They have circular DNA similar to that of bacteria.

23
Q

State where glycolysis occurs in a living cell

A

cytoplasm

24
Q

what is meant be a hexose sugar?

A

a 6 carbon sugar

25
Q

Explain why phosphorylation of glucose is necessary at the start of respiration.

A

To make glucose more reactive so that it is more likely to form TP

26
Q

Describe the role of NAD in glycolysis.

A

The coenzyme NAD is a hydrogen acceptor.

27
Q

State the products of glycolysis of one molecule of glucose.

A

Two molecules each of : pyruvate, reduced NAD and ATP.

28
Q

What happens to pyruvate when the cell has an adequate supply of free oxygen

A

It passes to the link reaction in a mitochondrion.

29
Q

Outline the role of coenzymes in the glycolysis pathway.

A

The coenzyme NAD accepts hydrogen atoms from the substrate molecules in stage 3 as triose phosphate is oxidised

30
Q

Explain how oxidation occurs during glycolysis although no oxygen is involved.

A

During stage 3, hydrogen atoms are removed from triose phosphate (and combine with NAD). This is an oxidation reaction.

31
Q

. Suggest how the structure of a mitochondrion from a skin cell would differ from that of a mitochondrion from the heart muscle tissue.

A

A mitochondrion from a skin cell would be smaller and have fewer cristae and shorter cristae in the inner membrane, because skin cells are not as metabolically active as heart muscle cells.

32
Q

Summarise the events of the link reaction.

A

Pyruvate enters the matrix of the mitochondria an is :

     Decarboxylated (carbon dioxide removed) 

     Dehydrogenated (hydrogen removed) 

     Combined with coenzyme A to give acetylcoenzyme A (ACoA)
33
Q

What is the role of coenzyme A in aerobic respiration.

A

It transfers an acetyl (2-carbon) group from pyruvate into the Krebs

cycle.

34
Q

State the role of oxygen in aerobic respiration.

A

The coenzyme NAD is a hydrogen acceptor.

35
Q

What is meant by chemiosmosis?

A

The production of ATP by a flow of protons through ATP synthase.

36
Q

Describe briefly how ATP is produced using the potential energy of a proton gradient

A

The inner membrane of the mitochondria is impermeable to Hydrogen

ions, they flow down their concentration gradient through protein

channels which span the bilayer. Part of the protein is the enzyme ATP

synthase which synthesises ATP.

37
Q

Explain why mature erythrocytes (RBC) cannot carry out the link reaction of Krebs cycle.

A

They do not have any mitochondria. Both of these reactions take place in the

mitochondrial matrix.
38
Q

Aerobic prokaryotes can carry out the link reaction, Krebs cycle and oxidative

phosphorylation. Suggest where in the prokaryotic cell these reactions take place.
A

Krebs cycle will occur in the cytosol (cytoplasm) and oxidative phosphorylation ion the cell

membrane which is likely to be invaginated (folded inwards) into structures called  

mesosomes.
39
Q

Explain why each stage of Krebs cycle needs to be catalysed by its own specific enzyme

A

Each substrate has a different molecular configuration so each step needs an enzyme with

a specifically shaped active site that is complementary in shape to the substrate

molecules.

40
Q

State the role of pyruvate dehydrogenase.

A

It removes hydrogen atoms from pyruvate.

41
Q

.Explain why oxygen is known as the final electron acceptor.

A

It combines with electrons at the end of the electron transport chain

42
Q

Explain how each of the following pieces of evidence supports the chemiosmosis theory:

lower pH in intermembrane space than in mitochondrial matrix.

A

The lower pH in the intermembrane space indicates a high concentration of hydrogen ions

 (protons).
43
Q

Name one cell in which : i) alcoholic fermentation occurs; ii) the lactate pathway occurs.

A

i ) Yeast ii) mammalian muscles

44
Q

Explain how reduced NAD from glycolysis is dehydrogenated in the absence of free oxygen

A

In alcoholic fermentation – ethanal accepts hydrogen from reduced

 NAD to form ethanol. 

 In mammalian muscles pyruvate accepts hydrogen from reduced NAD to  

 form lactate.
45
Q

List the disadvantages of anaerobic respiration in comparison with aerobic respiration .

A

Disadvantages – in both pathways wasteful products are produced that

 have chemical bond energy trapped in them. Lactate and ethanol is  

 toxic and restricts the use of the pathways. The ethanol pathway is  

 irreversible – the lactate pathway is reversible in the cori cycle.  

 There is a net gain of only 2 molecules of ATP per glucose molecule  

 during anaerobic respiration
46
Q

Describe the role of the Cori cycle in mammals.

A

It ‘resuses’ lactate and prevents the wasteful loss of some of its

 chemical bond energy . 

 It prevents a potentially disastrous fall in plasma pH.
47
Q

Define the term ’respiratory substrate’

A

A molecule from which energy can be liberated to produce ATP in a

 living cell.
48
Q

Name two respiratory substrates other than glucose

A

Fatty acids – carbon atoms are detached in pairs as ACoA and fed into

 the Krebs cycle; Amino acids are  deaminated and their carbon –  

 hydrogen skeletons converted into pyruvate or into ACoA.
49
Q

Explain why lipids have a greater energy value than carbohydrates.

A

Lipids have more hydrogens per molecule than have carbohydrates.

50
Q

When mammalian muscle tissues are rapidly using ATP they can regenerate it almost entirely by anaerobic glycolysis and lactate fermentation. A great deal of glucose is used but this process is not as wasteful as ethanol fermentation. Suggest why this is

A

Because lactate can be recycled back to pyruvate (which can be changed to acetate and enter Krebs cycle) or changes into glycogen and stored for later use. Ethanol is not recycled and is not respired by yeast.

51
Q

Why can’t mammalian tissues carry out alcoholic fermentation of pyruvate under

  anaerobic conditions.
A

They do not have the particular type of pyruvate decarboxylase that catalyses the change of pyruvate to ethanal.

52
Q

Explain how a build up of acid during glycolysis leads to muscle fatigue in mammals.

A

The lowering of pH interferes with hydrogen bonds and disrupts the tertiary structure of enzymes involved in muscle contraction.

53
Q

Suggest how diving mammals such as seals whales and dolphins can swim below water without suffering muscle fatigue.

A

They have buffers in their blood. Non-diving mammals also have buffers (molecules that can accept /donate hydrogen ions and so resist changes in pH) but diving mammals will have more or more efficient ones. Haemoglobin can act as a buffer. There are also chemicals such as dipotassium hydrogenphosphate and potassium dihydrogenphosphate.

54
Q

Explain why palmitic acid a large molecule can pass into the matrix of the mitochondria.

A

It is a fat so dissolves in the lipid bilayer of the mitochondrial membranes.