Photosynthesis Flashcards
What is photosynthesis?
Process by which plants, as well as some bacteria and protists, convert solar energy into chemical energy.
What is the chloroplasts most important pigment?
Chlorophyll
What are the pigments found in chloroplasts?
Chlorophyll
Carotenoids
Phycobilins
Color and structure of pigments found in chloroplasts.
Chlorophyll- green. Porphyrin rings containing mg at center. Includes chlorophyll a, b, c, d.
Insoluble in water
Carotenoids- red, orange, or yellow. Composed of 2 small six-carbon rings connected by a chain of carbons. Insoluble includes carotenes and xanthophylls
Phycobilins- water soluble found in stroma
What will be used to separate plant pigments
Chromatography paper
The rate at which pigments move friend on what?
Solubility of solvent and degree of attraction to paper
Polarity of products:
Chromatography paper
Carotenoids
Organic solvents
Chromatography paper is polar as it has cellulose
Organic solvent is non polar
Carotenoids non polar remain dissolved
Polar pigments get attracted to paper
What is retention factor (RF)?
Relates the traveled distance by a pigment to the distance traveled by the solvent during the chromatography process
Materials for activity 1: Identification of photosynthetic pigments by partier chromatography
Chromatography paper
Spinach leaf pigments extracted in acetone
Capillary tube
Chromatography solvent of petroleum ether and acetone
Formula for retention factor RF
Distance traveled by pigment/distance traveled by solvent
Rd=P/S
Materials activity 2a: the action spectrum for photosynthesis
Elodea leaves
Spinach leaves for absorption part
5 percent sodium bicarbonate
Cellophane paper( red yellow green blue violet)
How long do you need to wait for oxygen and how many watts?
45 min. 200 watts bulb
How many spinach leaves
10-15 in 200 mL
How much are you diluting the spinach extract?
9 mL water to 1mL extract
What wavelength setting spectrometer?
400 nm increasing 25 nm increments up to 725
