Phlebotomy Flashcards
Haima is the greek word for
Blood
3 types of blood vessels
veins
arteries
capillaries
The longest blood vessel is
Vein (D: 5mm)
The smallest and most numerous blood vessel
Capillaries (D: 8um)
Diameter of arteries
4mm
Blood is composed of (3) portions
Solid portion
Liquid portion
Gaseous portion
Liquid portion of unclotted blood
Plasma
Liquid portion of clotted blood
Serum
Cellular elements are otherwise known as
Hemocytes
The solid portion of the blood contains
RBC
WBC
PLT
Other names for RBC
Erythrocyte
Akaryocyte
Erythroplastids
Other names for WBC
Leukoplastids
Leukocytes
Granular WBCs
Basophil
Eosinophil
Neutrophil
Agranular WBCs
LYMPH
MONO
Other names for PLTS
Thrombocytes
Thromboplastids
pH of blood
7.35-7.45
Average specific gravity of blood
1.055
SG of water
1.000
Blood is thicker than water
3.5-4.5x
mL of blood per kg of body weight
75-85 mL
grams of solid per 100 mL of blood
20 g
Patient must be identified by asking for his/her
Name
Age/Sex
Birthdate
The most critical step in blood collection is
Patient identification
The mortal sin of phlebotomy
Mislabeling
Physiologic factors that can affect test results
Posture
Diurnal Rhythm
Stress
Exercise
Diet
Smoking
Shift in posture from supine to standing/sitting can increase the levels of
Protein
Cholesterol
Iron
In the morning the following are increased:
Cortisol
TSH
Iron
EO count is ____ in the morning
decreased
Fe, Chole, and Protein concentrations are increased when there is a shift in posture due to
them being too large and are not readily filtered into the the tissues from the blood vessels to the interstitial spaces
Stress increases
WBCs
Fibrinogen group
Fibrinogen group are thrombin-sensitive group because
they are all activated by thrombin
Factors activated by thrombin
1
5
8
13
Exercise can increase (9)
Crea, CK, TP, Myoglobin, AST, HDL-Chole, LDH, WBC count, PLT count
After a fatty meal, ___ is falsely increased; also ___-ALP
Hemoglobin
Intestinal ALP
Smoking increases
WBC count and cortisol
Skin puncture may be used for (5)
newborn, pedia
severely burned px
px veins reserved for tx purposes
extremely obese px
elderly px w/ fragile veins
For infants <1yo, the site for skin puncture is
medial or lateral side of the heel portion of the plantar surface of infant’s foot
For >1 yo and adults, the site for skin puncture is on the
Palmar surface of the non-dominant 3rd or 4th distal finger
Recommended depth for skin puncture for infants and small children
<2.0 mm
Depth of puncture for adults
2-2.5 mm
Too much pressure or milking/squeezing the site can cause
hemolysis
introduce excess interstitial fluid
Most common skin antiseptic
70% isopropyl alcohol
The first drop of blood must be discarded in skin puncture to
Discard excess tissue fluid/interstitial fluid
Discard dead epidermal cells
Facilitate free flow of blood
Devices used for skin puncture
Capillary tubes
Microtainer tubes
Order of draw for skin puncture
Tube for Blood Gas Analysis
Slides
EDTA
Other microtainer with anticoag
Serum microcollection tubes
First tube that is filled in skin puncture
Tube for blood gas analysis
First tube that is filled before other microcollection tubes
EDTA
This can increase the blood flow by 7x
Warming of the puncture site
How to warm the puncture site
Warm washcloth (40-42C) for 3-5 mins max
Most common site for venipuncture
Superficial veins of the antecubital fossa
Two anatomical patterns of the veins
H
M
H: veins used in order of preference
median cubital
cephalic
basilic
M: veins used according to preference
median vein
accessory cephalic
basilic
Important Considerations in Venipuncture:
Angle between skin and needle must be
<30
Important Considerations in Venipuncture:
Recommended length of time for tourniquet application
< 1 min/60 sec
Important Considerations in Venipuncture:
Effects of prolonged tourniquet application includes
Hemoconcentration
Hemolysis
Shortened coagulation time
When there is stasis caused by prolonged tourniquet application, these factors accumulate leading to falsely shortened clot-based coagulation test results
Factor VIII and VWF
Important Considerations in Venipuncture:
The tourniquet must be located _____ above the venipuncture site
3-4 inches/ 7.5-10 cm
Important Considerations in Venipuncture:
Most common needle size for adult
21 G
Length: 1 inch
The higher the gauge,
the smaller the bore size
Important Considerations in Venipuncture:
A phlebotomist must never puncture a patient more than
twice
Important Considerations in Venipuncture:
Pumping of fist during blood collection will result to an increase in
Potassium
Important Considerations in Venipuncture:
Sites to avoid
Veins of the inner wrist,
veins in the feet
fistula
arteries
inflamed sites
edematous sites
Important Considerations in Venipuncture:
Causes of hemolysis
Prolonged tourniquet application
Moisture/contamination in blood CT
Needles with too small bores
Excessive agitation
Frothing of the blood sample
Important Considerations in Venipuncture:
Standard Precautions was originally called
Universal Precautions
Practices to control diseases in which all blood, body fluids, and unfixed tissues are handled as if they were potentially infectious; including unfixed slides and microhematocrit clay
Standard Precautions
The most important practice to prevent the spread of infectious diseases
Hand washing
During hand washing, rub hands vigorously for at least
15 seconds
Important Considerations in Venipuncture:
Replace sharps container when they are
3/4 full
The most common cause of needle puncture or puncture from other sharp objects is
improper disposal
Bruise is aka
Ecchymosis
The most common complication encountered in venipuncture
Ecchymosis/Bruise
How is ecchymosis different from hematoma
Ecchymosis: leakage of SMALL amounts of blood, FLAT purplish discoloration
Hematoma: leakage of LARGE amounts of blood, SWOLLEN/BULGING purplish discoloration
To avoid hemoconcentration after prolonged tourniquet application, wait for ____ before reapplying tourniquet
2 mins