pharmaceutical and chemical industry Flashcards

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1
Q

List six major products/categories of applied microbiology, stating one example of
a microorganism and its associated product from the category

A
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2
Q

what is an antibiotic

A

compounds produced by microorganisms to inhibit the growth of other microorganisms

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3
Q

state and outline the method used to screen antibiotics.

A

the cross streak method
1 spread a soil dilution on the plate
2. incubate
3. overlay with an incubator organism and incubate
4. streak suspected antibiotic producer across on side of the plate
5. incubate to permit growth and antibiotic
6. cross streak with test microorganism
7. incubate to permit test microorganism growth.

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4
Q

5 bacteria with the ability to produce antibiotics and the name of the the antibiotic

A

bacitracin- bacillus licheniformis
erythromycin - streptomyces erythreus
penicillin- penicillin chrysogenum
streptomycin- streptomyces griseus
lincomycin- streptomyces lincolnensis

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5
Q

which bacteria produces a human protein and name the protein

A

human insulin produced by a recombinant E.coli strain

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6
Q

which bacteria produces vitamin B-112

A

Propionibacterium
freudenreichii and Pseudomonas denitrificans

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7
Q

how is riboflavin of vitamin B2 produced

A

It is a by-product of acetone-butanol fermentation and
is produced by various Clostridium sp

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8
Q

amino acids and the bacteria that produces them

A

lysine- Brevibacterium flavum
glutamic acid - Corynebacterium glutamicum, Brevibacterium sp
aspartic acid and phenylalanine- Brevibacterium
and Corynebacterium
(aspartame)
gluconic acid- Acetobacter sp (bacteria) Penicillium and Aspergillus sp

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9
Q

fungi used commercially

A

for production gluconic acid Penicillium and
Aspergillus sp

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10
Q

amino acids and their uses

A

glutamic acid as a flavour enhancer in msg
aspartic acid and phenylalanine (aspartame) sweetners
lysine used for human and animal feed

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11
Q

which bacteria produces lactic acid and list 5 uses

A

produced by lactobacillus sp
* Preservatives in foods
* In leather industry for deliming hides
* Textile industry for fabric treatment
* Various forms of lactic acid have been used in resins, plastics,
electroplating
* Making baking powder
* Animal feed supplements
* Dairy products

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12
Q

sorbose is used in the production of

A

Sorbose is used in the manufacture of
ascorbic acid (vitamin C)

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13
Q

what are proteases

A

class of enzymes that attack the peptide bonds of
protein molecules, forming small peptides

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14
Q

commercial use of proteases

A

e laundry industry, principally in making
detergents

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15
Q

what is an advantage of using proteases in detergents

A

the use of non-phosphate
laundry detergents that function at lower wash
temperatures has led to increasing incorporation of
enzymes into liquid and powdered detergents to
improve their cleaning performance

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16
Q

which bacteria produces proteases

A

Bacillus licheniformis

17
Q

how does protease work as a detergent

A

enzyme degrades various
proteinaceous materials, forming small peptide
fragments These fragments can be washed out readily

18
Q

what are the commercial uses of amylases

A

making sizing (agents used to fill pores in cloth and paper)
* removal of starch sizing from woven cloth
* production of high fructose syrup, that is used to sweeten juices, soft drinks

19
Q

bacteria and fungi that produces amylases

A

Bacillus sp Aspergillus sp

20
Q

bacteria that produces xanthan and uses

A

Xanthomonas campestris
Used as a thickener in foods such as dairy products and salad dressings; and cosmetics such as cold creams and shampoos

21
Q

review these other ,icroorganisms and their uses

A

Baker’s yeast—S. cerevisiae
▪ Peat moss—contains Rhizobium and
Bradyrhizobium for N2
fixation
▪ Insect toxin (Bt-toxin)—Bacillus thuringiensi