Pecori 2022 Apobec Functions Flashcards
A3a functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- Rna and dna in viruses and hosts
- N and C
- innate immune cells - macros monos microglia and dendritic cells
- 5-TC-3
- protection form viruses
A3g functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host rna and viral rna&dna
- cytoplasm
- all over - breasts, reproductive organs, IFNa activates cells, bone marrow
- 5-CC-3
- protection from viruses and retro elements
A1 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host rna&dna and viral rna
- N and C
- GI tract in human and mouse and immune cells in mice only
- 5’AC’3
- cholesterol metabolism, monocyte transcriptome (ms only), antiviral
Aid functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ssDNA of hosts
- N and C
- germinal center B cells, lymphoid tissue
- 5-wrc-3
- secondary antibody diversification
A2 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host dna ss/Da binding
- N and C
- muscle and some B/T cells
- ND
- ND
A3b functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- dna of viruses and hosts
- nucleus
- ifna activated liver cells, bone marrow, gi tract, kidney and bladder
- 5-TC-3
- protection from viruses
A3c functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Nucleus and Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and lots more
- 5-TC- 3
- protection from viruses
A3d functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and more
- 5-TC- 3
- protection from viruses
A3f functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and more like ifna cells
- 5-TC- 3
- protection from viruses
A3h functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm and nucleus
- peripheral blood cells, bone marrow and lymphoid tissue
- 5-TC- 3
- protection from viruses
what are the generalist apobecs?
a3a a3g and a1
what part of generalists determines substrate differentiation?
loops 1 3 5 7 determine binding and single AAs determine RNA and or DNA binding ability
what structure do generalists all have in common?
they all have a ‘u’ shaped groove where substarte binding occurs
which apobecs have nuclear localization signal domains?
aid a1 and a3b
which apobecs have nuclear export signal domains?
aid and a1