Epitranscriptome and A1 editing - Karen Bulloch

Question 1

Apobec1 has 2 cofactors

Answer 1

RBM47 and A1cf

Question 2

ADARs =

Answer 2

adenosine deaminases that act on RNA

PS
ADARs = imp for brain (and immune system) ALS example (7)

Question 3

A1-/- animals exhibit ….

Answer 3

retrotransposon mobilization later in life that lead to cell death

Question 4

A1 Editing frequency is r/t

Answer 4

expression

Question 5

A1 edits (3 cell types in order)

Answer 5

macropahges (#1) and enterocytes (#2) and dendritic cells (#3)

Found by (sanger (from pcr) and sqPCR)

Question 6

A1 modifies CD36 receptor on BMDMs. This causes….

Answer 6

decreased bioavailability of the receptor

(Flow and Luciferase assay – add luciferase onto end of gene of interest so if its produced you see a signal if its mutated you don’t)

Question 7

A1 edits and reduces transcript abundance that typically occur in ____ of transcripts

Answer 7

3’ UTR

Question 8

A1-/- mice do not exhibit differences in _____ compared to controls

Answer 8

mRNA amounts of any gene

Question 9

A1 edits in RNA don’t affect the the RNA’s _______

Answer 9

stability/ amount of RNA that is present after editing event occurs

Question 10

Biochemical fractionation used to find editing differences in chromatic ass. Nascent transcripts were not edited more in ________________

Answer 10

nucleus or cytoplasm

Question 11

They used luciferase cassettes (Renilla and firefly to standardize) to look at

Answer 11

3’utr expression

Question 12

RBM47 causes what type of rna editing - codon changes and what is produced as a consequence?

Answer 12

Caa to Uaa which produces a stop codon

Apob48 and apob100

Question 13

A1 target specificity is determined by what?

Answer 13

The 2 cofactors - rbm47 and a1cf have different mRNA edited transcripts