PCV

Question 1

% of total volume of blood sample

Answer 1

PCV

Question 2

Methods to estimate PCV

Answer 2

Macromethod-wintrobe tips
Micro-capillary tube
Automated

Question 3

Wintrobes method

Answer 3

More blood than micromethod

Question 4

Principle of wintrobe

Answer 4

Anticoagulated blood
-centrifuged
-optimum,speed radius duration
-RBC-bottom and plasma on top
-ratio of settled cell in% is calculated as hematocrit

Question 5

Method of wintrobe

Answer 5

-blood in EDTA in tube and mix
-fill with Pasteur pipette to avoid bubbles
-tip of pipete gradually withdrawn from tube by simultaneous expulsion of blood

Question 6

Points to note in wintrobe

Answer 6

-hemolysis blood is prevented
-upto 10 mark
-centrifuge at 300 rpm at 30 min
-stop after exact time, mark reading

Question 7

Layers after centrifuge in wintrobe

Answer 7

Lower-packed red cells
Middles-buffy coat (thin greyish)-wBC and platelets
Upper-plasma layer -straw coloured

Question 8

Colour change in wintrobe

Answer 8

Red color-hemolysis ,faulty technique/wet syringe
Pale-red in heamolytic anemia ,deep yellow in jaundice milky-lipeamia
Cloudy-nephrotic syndrome

Question 9

Normal value

Answer 9

Adult male-40 to 54%
Famales-36 to 47%
Birth-53 to 68
I yr 35%

Question 10

Uses of PCV

Answer 10

To detect presence or absence of anemia or polycythemia
To estimate red cell
To check the accuracy of hemoglobin value

Question 11

Increased conditions in pCV

Answer 11

Polycythemia,burns,shocks,dehydration

Question 12

Decreased condition in PCV

Answer 12

Chronic obstructive pulmonary disease
Anemia
High altitude conditions