Buffy Coat Flashcards
About usual volour and change of colour
Usually-Grey
Green -if sample contains neutrophils in large amounts
No of WBC and platelets determine
Thickness of buffy coat
Normal thickness
0,5 -1mm
O.1 mm corresponds to
1000WBC/ml
Increase and decrease of thickness
Increases in leucylosis,increase in platelets
Decrease in leucopenia
Uses.buffy coat used to extract DNA from blood of mammal.why?
Smears from buffy used to?
No DNA in mammalian RBCand nucleus
Smears-demonstration of lupus erythematosus(LE)cells,malaria parasites,immature cells
Buffy coat smear
Defibrinated blood into wintrobe/narrow testube/capillary tube hematocrit tube by Pasteur pipett
-centrifuge tube for 15 min at 3000 rpm
-remove supernatanat serum with fine pipette and save Buffy after centrifugation
-remove Buffy coat by Pasteur pipette
-in a drop of patient plasma emulsify the buffy coat then make smears
-blood dry in air
-fix in methanol
-stain with leishman stain
Buffy coat useful for
Detection of bacteria,fungi/parasites within neutrophils,monocytes,/circulating macrophages
Micro hematocrit
Small heparin capillary tube with uniform thickness is used
Requirements of microhematocrit
Capillary HCL tube
Disposable glass tube-7 cm in length and diameter 1mm
Midrohematocrit centrifuge
Plane or heparinized blood
-Blood filled 2/3rd of tube by capillary action
-centrifuge in high speed with special head to hold the tube in horizontal position
-to hold in place and minimize breakage, special robber pudded rim
-placed as sealed end to periphery
-centrifuge at 12000 for 5 min
-remove tube,read result using cord reader of micro hematocrit
Advantage and disadvantge of microhematocrit
- easy to rapid,convenient
Small quantity of blood
Less time
Capillary blood from skin puncture can be used
*require costly equipments
