PCR types Flashcards
1
Q
what is the main benefit of qPCR?
A
offers you timed snapshots of each cycle of PCR.
2
Q
What is Taqman?
A
A fluorescent probe used in qPCR
3
Q
What do Taqman probes contain on their 3’ and 5’ end?
A
3’ end: quencher
5’ end: fluorophore
4
Q
Why is Taqman superior to intercalated dye in qPCR?
A
TaqMan is specific to a target sequence, so no non-specific fluorescence.
