PCR Steps Flashcards
1
Q
- Denaturation
Temp raised to _
A
Break hydrogen bonds
2
Q
- Annealing
Mixture cooled so _ can be attached or annealed to ends of single strands
A
Short sequences of dna
(Called primers)
3
Q
- DNA synthesis
An enzyme called polymerase takes bases from mixture and _
A
Adds them to each single strand
(Making double strands)
