PCR gel electrophoresis Flashcards
what are the 4 basic steps using PCR in viral diagnostics
collect sample
extract genetic material
amplify viral sequence
visualize results
what does PCR stand for
Polymerase chain reaction
What does PCR allow you to do
It allows you to copy DNA and replicate specific genes of a virus
what is the name of the enzyme used in PCR
reverse transcriptase
why is reverse transcriptase used
this turns RNA to DNA before copying it
Agargose
Used to separate DNA segments
TAE buffer does what
this allows electrical current flow
Loading dye
This helps to see how far it goes into gel.
Helps sample be thicker
Helps to fall into well easier
DNA ladder/ marker
help to decide the size of the results
wells in agarose gel
where you load the dye
what does a line farther down the gel indicate?
A line at the lower part of the gel indicated that the DNA fragments were smaller
what does a line of DNA fragments close to the top of the ladder mean?
This means that the DNA fragments were larger if closer to the top of the ladder.
