elisa study guide Flashcards
what is an antigen
a foreign substance that will trigger an immune response
what is an antibody
Specific defense mechanisms produced by b-cells
what does ELISA stand for
Enzyme linked immunoabsorbant assay
which one allows you to detect serum antibodies
antibody test
which one allows you to detect antigen (which would be from the microbe itself)
antigen test
which one did we use in the lab to detect antibodies to HIV
antibody test
known antigen =
sample of hiv
Serum antibody=
from blood sample, recognizes antigen if present
secondary antibody
recognizes human antibodies
chromogen
substrate color change
what is the purpose of the enzyme linked to the secondary antibody
to make the color
what is the purpose of the chromogen linked to the secondary antibody
to visualize a positive result
what does a positive result look like
it would have all 4 components on the graph in order to be a positive reaction
why is it important to wash after each step
to get rid of everything that does not bond
does a positive elisa test mean the microbe is still present?
no, this is because this test only shows you if the microbe has EVER been present, not if it is currently present.
