PCR Flashcards
1
Q
In vivo gene cloning
A
transferring to a host cell using a vector, uses a cell
2
Q
in vitro gene cloning
A
using PCR outside of a cell
3
Q
PCR
A
polymerase Chain Reaction
4
Q
What is PCR
A
a method of copying fragments of DNA. An automated process
5
Q
what effect does PCR being automated have
A
It makes it more rapid and efficient than using a vector
6
Q
What is needed for PCR to occur
A
- The DNA fragment to be copied
- DNA polymerase
- Primers
- Nucleotides
- THermocycler
7
Q
What is DNA polymerase used for
A
it joins together nucleotides. For pcr we use taq polymerase
8
Q
What is a taq polymerase
A
polymerase taken from bacteria that live in hot springs so are thermostable
9
Q
thermostable
A
tolerant to heat
