Pcr Flashcards
1
Q
Step 1
A
Dna is HEATED at approx 90degrees
Causing DNA to denature and strands to seperate.
2
Q
Step2
A
DNA is cooled to approx below 60degrees.
This makes short primers to bond to the seperate DNA strands
3
Q
Step 3
A
The DNA is heated again to over 70degrees.
When heat-tolerent DNA polymerase is used to synthesise New strands from nucleotide
4
Q
Sequence specific primers
A
These are designed by the scientist and can be manufactured by a machine
5
Q
What does pcr do
A
PCR allows specific sections of DNA to be amplified in vitro
