PCR Flashcards
1
Q
What is PCR?
A
PCR is a technique that replicates short sections of DNA quickly
2
Q
Why is DNA heated to between 92 and 98 degrees?
A
To seperate strands and break weak hydrogen bonds
3
Q
Why is DNA cooled to between 50 and 65 degrees?
A
It is cooled to allow the primer to bind to its target sequence
4
Q
Why is DNA heated to between 70 and 80 degrees?
A
For heat-tolerant DNA polymerase to replicate the region of DNA
5
Q
What can PCR be used for?
A
Personalised medicines, DNA profiling, forensically, paternity testing or to analyse disease risk
