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General Zoology Lab > Parts of the Microscope > Flashcards

Parts of the Microscope Flashcards

1
Q

These are the 3 main structural part of the microscope.

A

Head, Arms, Base

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2
Q

Contains the microscopic illuminator

A

Base

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3
Q

Contains optical components such as the eyepiece and objective lenses.

A

Head

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4
Q

Connects the base to the head and supports the eyepiece tube. It’s where you carry the microscope.

A

Arms

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5
Q

Turns the lamp on and off

A

Power switch

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6
Q

Consists of a lamp bulb and one or more lenses to produce a cylindrical beam of light directed toward the base of the condenser. May have a ground glass and may have an iris or field diaphragm.

A

Illuminator

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6
Q

Controls the brightness of the lamp. On most microscopes it is a continuously variable rheostat, controlled by a sliding
switch

A

Illumination control

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7
Q

A frosted sheet of glass that scatters the light from the lamp bulb to provide more diffuse light.

A

Ground glass

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8
Q

Controls the diameter of the illuminating beam of light as a guide to focus the light from the condenser onto the specimen.

A

Iris Diaphragm

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8
Q

Contains a set of lenses that focus the light on the specimen.

A

Condenser

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9
Q

The condenser has the following components:

A
  1. Focusing knob
  2. Front lens
  3. Iris Diaphragm
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10
Q

The glass surface closest to the specimen. Be careful not to touch this
surface as it is easily scratched

A

Front lens

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10
Q

Controls the aperture of the illuminating light and used to adjust contrast.

A

Iris diaphragm

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10
Q

Moves the condenser up and down to adjust the focus of the light on the specimen

A

Focusing knob

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11
Q

Holds several objective lenses that can be rotated into position to change the lens

A

Revolving nosepiece

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12
Q

Create a magnified image of the specimen.

A

Objective lenses

13
Q

There are 4 types of objective lenses:

A
  1. Scanning Lens
  2. LPO
  3. HPO
  4. OIO
14
Q

Used to get an overview of the structures present in a section and to find areas for more detailed observation.

A

Scanning lens

15
Q

The most useful magnification to identify tissues

A

Low Power Objective (LPO) 10x lens

16
Q

Used to see the details of cell and tissue organization.

A

High Power Objective (HPO) 40x lens

17
Q

Because it requires the use of immersion oil, is used primarily to see subcellular details.

A

Oil Immersion Objective (OIO) 100x lens

18
Q

Forms an image that can be visualized by the eye or a camera. In a binocular
microscope, the distance between the two tubes can be adjusted to fit the distance between the observers’ eyes.

A

Eyepiece

19
Q

Used to raise and lower the specimen stage to focus the image of the specimen. It consists of the coarse and fine focus.

A

Focusing controls

20
Q

Used to focus the specimen at 4x and 10x

A

Coarse focus

21
Q

Used to focus the specimen at 40x and 100x, but only after initially focusing at lower magnification.

A

Fine focus

21
Q

Holds the microscope slide. Consists of the slide holder and slide holder travel controls.

A

Specimen stage

22
Q

Spring-loaded device to hold the microscope slide in place on the stage.

A

Slide holder

23
Q

Allow the slide to be moved along two axes: longitudinal and lateral.

A

Slide holder travel controls

24
Q

The total magnification for the microscope is obtained by multiplying the:

A

Magnification of the eyepiece and magnification of the objective lens

25
Q

___ is the ability to enlarge an image (what you see looking through the eyepiece).

A

Magnification

26
Q

___ might be monocular or binocular.

A

Ocular lenses

26
Q

Ocular lens may be ___, ___, ___.

A

5x, 10x or 30x

27
Q

The area your lens can see; the actual “circle” you see. The circular area will ___ as you increase the magnification.

A

Field of view, decrease

28
Q

The thickness of the vertical range in which the specimen remains in focus. It decreases as the magnification ___.

A

Depth of focus, increases

29
Q
  • Light illuminated
  • Image is 2D
  • High magnification, low resolution
  • Mechanic focusing
  • Good for transparent or translucent specimens
A

Compound microscope

30
Q
  • Light illuminated
  • Provides 3D image of specimens
  • Low magnification (5x - 10x)
  • Mechanical focusing
A

Dissection Microscope

31
Q
  • Uses electron illumination
  • 3d view of image
  • High magnification, high resolution
  • Electrical focusing
A

Scanning Electron Microscopy (SEM)

32
Q
  • Uses electron illumination
  • 2d view (internal structures)
  • High magnification, high resolution
  • Electrical focusing
A

Transmission Electron Microscopy (TEM)

33
Q

___ have shorter wavelengths than light.

A

Electrons

General Zoology Lab (3 decks)

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