Part 2.2 Flashcards
Other Tests:
_____: test for the presence of Antinuclear Antibody
- neutrophil or macrophage that has phagocytized (engulfed) the denatured nuclear material of another cell
- formation is characterized by the production of a number of _____
- Demonstration of LE cells therefore suggests the presence of these antinuclear antibodies also termed as ______
Procedure:
1. ______ is collected
- allowed to clot for __ hour at 37°C or,
- for ___ hours at room temperature
2. macerated sample is collected in 3-4 _____
- to be centrifuged at ____ rpm for ____ minutes
LUPUS ERYTHEMATOSUS (L.E.) CELL PREPARATIO
- LE CELL
- AUTOANTIBODIES
- LE FACTOR
Procedure:
1. 10-15 mL of non-anticoagulated whole blood
- 1he
- 2hrs
2. Wintrobe tubes
-2550 rpm for 30 minutes
cont for LUPUS ERYTHEMATOSUS (L.E.) CELL PREPARATION
- ## for LE cell to occur (3)-
- - After centrifugation, the _____ is collected to prepare a smear
- using ____ method - LE factor causes ____ (lysed nucleus becomes a homogeneous amorphous
mass) - when damaged nucleus is surrounded by several neutrophils
- this is not considered as ____ - other antinuclear antibody test that replaces LE test
- uses
- for LE cell to occur (3)
- LE factor
- extruded nuclei
- phagocytic neutrophil - buffy coat
- wedge method - nuclear lysis
- Rosette formation
- positive - Fluorescent test
- fluorescein-conjugated anti-human IgG
_____: a study where PBS is collected first to avoid causes of physical and emotional stress
- collection for adults
- collection for children
BONE MARROW STUDY
- posterior iliac crest; sternum
- tibia
BONE MARROW STUDY
______: taken before aspiration biopsy to avoid any disruption of marrow architecture
- uses _____ which allows the collection of core
- prepared by touching the specimen on a slide; or touch preparation
- fixative
TREPHINE BIOPSY
- jamshidi needle
- Imprint biopsy
- 5% Zenker fluid
BONE MARROW STUDY
____: follows immediately trephine biopsy
- uses what needle
- ___ mL of marrow using a 30-35 mL syringe
- _____ to prevent morphological distortion essed within
ASPIRATION BIOPSY
- University of Illinois Sternal needle
- 1-3mL
- mixed EDTA
BONE MARROW SMEAR PREPARATION
- compensates for
hypocellular marrow and allows for examination of large numbers of nucleated cells without interference from fat or RBCs - Prepared after the centrifugation of sample in a Wintrobe tube at ___ for 8-10hrs
- Fixative (3)
- stain (3)
- Buffy coat (concentrate) smear
- 2800 rpm for 8-10 hours
- 10% formalin, Zenker glacial acetic acid, or B5
fixative - Romanowsky; iron stain; H and E
NORMAL MARROW CELLS
- Waterbug or comet appearance
- Important in bone resorption
- with iron storage
- Osteoblasts
- Osteoclasts
- Macrophages / Histiocyte
_____: Percentage of marrow space occupied by hematopoietic cells
- hypercellular disease
- hypocellular disease
Bone Marrow Cellularity
- polycythemia vera
- aplastic anemia
_____: At least 500 cells are counted; preferably 1000 cells, 500/slide (2 slides )
- Include all nucleated hematopoietic cells except ____ and ___
Bone Marrow Differential
- megakaryocytes and macrophages
M:E Ratio
- Myeloid:Erythroid (M:E) ratio
- ___ precursor : ____ precursor
- 2:1 - 4:1
- Granulocytic precursors:erythroid precursors
SMEAR PREPARATION FOR MALARIA EXAMINATION
- identification and determination of parasitemia
- fixed with ___ first them stained - For screening purposes
- first ____, air dride then stained
- stains for malaria blood smear
- by adding ___, you are dehemoglobinizing
- THIN BLOOD SMEAR
- methanol - THICK BLOOD SMEAR
- dehemoglobinized
- Wright’s; Giemsa
- distilled water
OTHER METHODS:
- 2 methods that uses 3.8% sodium citrate
- 2 methods that uses 5% sodium citrate
- uses 1.3% sodium oxalate
- uses heparin
- Graphic-Cutler Method
- Linzenmeier Method
2. - Micro-Landau Method
- Smith Micro Method
- Bray’s Method
- Roarke-Ernstene Method
