Parasitology - Blood and Tissue Protozoa (Malaria) Flashcards
Blood and tissue protozoa: Malaria species Pathogenesis, epidemiology, diagnosis, treatment
Name some species in the Sporozoa group.
- Plasmodium falciparum
- P.vivax
- P.malariae
- Toxoplasma gondii
- Isospora belli
- Cryptosporidium parvum
- Cyclospora cayetanensis
Briefly describe Protozoa.
- Single-celled eukaryotic organisms that range in size from 2-100 μm and can have various organs to aid motility, such as pseudopods, flagella, and cilia.
- They can reproduce sexually via fusion of gametocytes, or asexually by binary fission.
Some are pathogenic to humans and multiplication in the host can be controlled by either the immune system or drugs. The disease can be severe in the immunocompromised.
Protozoa are most prevalent in the tropical and sub-tropical areas.
What are the two main stages in the lifecycle of most protozoa?
- Trophozoite: actively feeding and multiplying stage.
- Cyst: infectious, protective, quiescent stage.
What main species are medically important blood and tissue Protozoa?
- Plasmodium spp.
- Babesia spp.
- Toxoplasma spp.
- Sarcocystis spp.
- Acanthoamoeba spp.
- Balamuthia spp.
- Naegleria spp.
- Leishmania spp.
- Trypanosoma spp.
What are the five species that can cause malaria?
- Plasmodium vivax
- P.ovale
- P.malariae
- P.falciparum
- P.knowlesi
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Charles Louis Alphonse Laveran (18 June 1845 – 18 May 1922) was a French physician. What did he discover?
The cause of malaria is a protozoan.
Sir Ronald Ross (13 May 1857 – 16 September 1932) was an Indian-born British doctor who received the Nobel Prize for Physiology or Medicine in 1902. What did he discover?
He found the malarial parasite in the gastrointestinal tract of the Anopheles mosquito, which lead to the realisation that malaria is transmitted by the Anopheles fly, specifically female.
In 2015, approximately __ ______ people – nearly half of the world’s population – were at risk of malaria. Most malaria cases and deaths occur in ___-_______ ________. However, Asia, Latin America, and, to a lesser extent, the Middle East, are also at risk. In 2015, __ countries and territories had ongoing malaria transmission.
Some population groups are at considerably higher risk of contracting malaria, and developing severe disease, than others. These include infants, children under _ _____ of age, _______ women and patients with ___/____, as well as non-immune migrants, mobile populations and travellers. National malaria control programmes need to take special measures to protect these population groups from malaria infection, taking into consideration their specific circumstances.
In 2015, approximately 3.2 billion people – nearly half of the world’s population – were at risk of malaria. Most malaria cases and deaths occur in sub-Saharan Africa. However, Asia, Latin America, and, to a lesser extent, the Middle East, are also at risk. In 2015, 97 countries and territories had ongoing malaria transmission.
Some population groups are at considerably higher risk of contracting malaria, and developing severe disease, than others. These include infants, children under 5 years of age, pregnant women and patients with HIV/AIDS, as well as non-immune migrants, mobile populations and travellers. National malaria control programmes need to take special measures to protect these population groups from malaria infection, taking into consideration their specific circumstances.
According to the latest WHO estimates, released in December 2015, there were ___ _______ cases of malaria in 2015 and ___,____ deaths.
___-________ _______ continues to carry a disproportionately high share of the global malaria burden. In 2015, the region was home to __% of malaria cases and __% of malaria deaths.
In areas with high transmission of malaria, children under _ are particularly susceptible to infection, illness and death; more than two thirds (70%) of all malaria deaths occur in this age group.
According to the latest WHO estimates, released in December 2015, there were 214 million cases of malaria in 2015 and 438 000 deaths.
Sub-Saharan Africa continues to carry a disproportionately high share of the global malaria burden. In 2015, the region was home to 88% of malaria cases and 90% of malaria deaths.
In areas with high transmission of malaria, children under 5 are particularly susceptible to infection, illness and death; more than two thirds (70%) of all malaria deaths occur in this age group.
What are the symptoms of malaria?
- Infections can be either asymptomatic or result in clinical symptoms. The incubation period is usually 10-15 days, but can appear years later in latent infections.
- Influenza-like symptoms: fever, sweats, headache, muscle pain, diarrhoea, vomiting.
- Untreated can become life-threatening: breathing problems, seizures, organ failure, severe anaemia, falciparum most severely can block blood vessels.
Outline the stages of reproduction of Plasmodium spp. (including both sexual and asexual stages)
- Sporozoites are injected into the bloodstream of a human through an infected female Anopheles fly (salivary glands).
- The sporozoites first infect hepatocytes.
- Over the next 5-10 days the parasite differentiates and multiplies inside the hepatocytes. 20,000 - 40,000 merozoites are released into the blood.
- These invade the red blood cells.
- During the intraerythrocytic stage the merozoites develop and multiply over the next 48 h. When the RBCs burst 15-32 merozoites are released.
- A small proportion of infected RBCs have parasites undergoing differentiation into either male or female gametes (which can be taken up by blood meal).
- In the insect mid-gut the male and female gametes fuse to form a zygote, which differentiates and grows into sporozoites in the salivary gland of the mosquitoes.
What are the stages of development of malaria in erythrocytes? [5]
- Ring stage
- Early trophozoite
- Trophozoite
- Schizont
- Merozoites
What disease does Plasmodium vivax cause?
Benign tertian malaria
Outline the main features of P. vivax.
Plasmodium vivax only invades young immature erythrocytes which enlarge and contain pink granules (Shuffner’s dots).
Has an incubation period of 10-17 days, which is followed by influenza-like symptoms with headache, muscle pains, photophobia, anorexia, nausea and vomiting.
- Have ring-shaped mature trophozoites
- Amoeboid mature trophozoites
Outline the pathogenesis of Plasmodium vivax.
Plasmodium vivax causes benign tertian malaria.
After infection and invasion of erythrocytes, increasingly more erythrocytes are ruptured in order to liberate merozoites and toxic cellulr debris (haemoglobin), which leads to typical patterns of chills, fever and malarial rigours (paroxysms (sudden recurrence)) appearing periodically (48 hourly).
This can progress to severe attacks concominant with the period of infection, replication, and cell lysis. if left untreated then chronic P.vivax infections last for years and can lead to brain, kidney, and liver damage.