Papers: Aims and techniques Lecture 5 Flashcards

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1
Q

What was the title and Aims of the Amaya paper?

A

“A role for FGFs in mesoderm induction”

Identifying the result of inhibiting FGF signalling in mesoderm formation `

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2
Q

What was the progression of experiments of the Amaya paper?

A
  1. In situ hybridisation of inhibited FGF signalling in 3 day old embryo (somites, notochord)
  2. FGF signalling required for mesoderm formation, 3 constructs
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3
Q

What was the title and aims of the Shulte-Merker and smith paper?

A

“Mesoderm formation in response to Brachyury requires FGF signalling”

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4
Q

[basic] Progression of experiments of Sulte-Merker and Smith?

A
  1. Ability of brachyury to induce mesoderm in animal explants requires FGF signalling
  2. Brachyury activates FGF transcription
  3. FGF signalling activates brachyury transcription
  4. FGF signalling maintains brachyury transcription
  5. FGF maintains activin/nodal induced brachyury expression
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5
Q

What was the title and aims of the Branney paper?

A

“Characterisation of the fibroblast growth factor dependent transcriptome in early development”

Identify the other targets of of FGF signalling in the mesoderm - Microarrays, a massively parallel approach to gene expression

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6
Q

[basic] Progression of the Branney paper?

A
  1. Analysed expression of 14000 genes in WT and FGFR dom. neg. embryos in a microarray
  2. Validation of samples
  3. Microarray scatterplot
  4. Validation of results
  5. Location of DUSP/MKP1/3 and result of dom.neg FGFR
  6. DUSP5/MKP1/3 inhibit FGF signalling
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