Papers: Aims and experiments - Lecture 1

Question 1

What was the aim of the Rudnicki Paper?

Answer 1

MyoD or Myf5 is required for the formation of skeletal muscle
Do myoD/Myf5 compensate for each other in skeletal muscle development?

Question 2

[brief] Progression of experiements in Rudnicki paper

Answer 2

1. Generated MyoD/Myf5 knockouts
2. Expected genotypes from the MyoD/Myf5 heterozygote cross
3. Norhtern blot of genotypes radioactively labelling myogenic genes
4. Immunohistochemistry with anti a-actin, anti-desmin with hetero-homo- MyoD/Myf5 genotypes
5. Hisology of Diaphragm/Intercostal muscles with hetero-homo- MyoD/Myf5 genotypes

Question 3

What was the title and aim of the Hasty paper?

Answer 3

“Muscle deficiency and neonatal death in mice with a targeted mutation in the Myogenin gene”

K/O myogenin, observe effects

Question 4

[brief] Progression of experiments in Hasty paper

Answer 4

1. Histology of the tongue

2. Northern blot of contractile protein genes and MyoD