Paper Chromatography Flashcards
What are the two phases of chromatography?
A mobile phase
A stationary phase
In chromatography what is the mobile phase?
Where the molecules can move. always liquid or gas
In chromatography what is the stationary phase?
Where the molecules can’t move. solids or thick liquid
What is the equation to work out the Rf value?
Rf = distance traveled by substance / distance traveled by solvent
What is the result of chromatography analysis called?
chromatogram
What is the Rf value?
The ratio between the distance traveled by the dissolved substance and the distance traveled by the solvent
Why is chromatography carried out?
To see if a certain substance is present in a mixture
What happens to the Rf value when the substance goes further through the stationary phase?
You get a larger Rf value
Why is the Rf value dependant on the solvent?
If you change the solvent the Rf value for a substance will change
In paper chromatography what is the stationary phase?
The chromatography paper
In paper chromatography what is the mobile phase?
The solvent
In paper chromatography, the amount of time the molecules spend in each phase depends on what two things?
- How soluble they are in the solvent
2. How attracted they are to the paper