Paper Chromatography

Question 1

Observayion

Answer 1

Violet spots

Question 2

Conclusion about the composition of sweat

Answer 2

Sweat have amino acids that interact with paper.

Question 3

Why changing solvent would result to a different RF value

Answer 3

Because every solvent has different polarities that dissolves to different subdtances

Question 4

Explain: chromatogram long and can’t find solvent front

Answer 4

Travel too far, run of the plate, can’t find solvent front; unknown solvent front, can’t calculate rf

Question 5

Explain: starting line ballpoint

Answer 5

Contaminate plate because of pigment; pen ink highly soluble in most solvents, drag the ink on and off the plate

Question 6

Explain: forget developing chamer

Answer 6

Will not absorb enough solvent because of evaporation

Question 7

Explain: mistakenly use chloroform in place of absolute ethanol

Answer 7

They have different polarity and solubility; different compounds, different separation patterns resulting to wrong results