Paper Chromatography Flashcards
chromatography
Chromatography is a chemical analysis technique used to separate substances in a mixture.
What is paper chromatography used for?
To separate a mixtures of soluble substances in liquids
What is the name given for the pencil line?
The baseline
Why should we use pencil for the baseline rather than pen?
Pen ink would dissolve in the solvent and move up the paper
Should the baseline be submerged in the solvent?
no
What are some solvents commonly used in chromatography?
water, ethanol
What is the name of the resulting paper we end up with?
A chromatogram
The ‘mobile phase’ refers to the molecules that can move.
Which is the mobile phase in paper chromatography?
the solvent
A substance which is more soluble in the mobile phase…
A substance which is more soluble in the mobile phase will spend more time in the mobile phase. This means it will move faster, and travel a further distance up the paper.
The ‘stationary phase’ refers to the molecules that can’t move.
Which is the stationary phase in paper chromatography?
the paper
A substance which is less soluble in the mobile phase…
A substance which is less soluble in the mobile phase will spend more time in the stationary phase, so move slower, and not travel very far up the paper.
The formula to calculate the Rf value:
distance travelled by the substance / distance travelled by the solvent
The steps of simple distillation:
- A solution is heated, usually using a Bunsen burner.
- The liquid in the mixture evaporates into a gas.
- The gas is cooled by a water jacket, and condenses into a liquid, which then flows into the beaker.
Fractional distillation can separate a mixture of several liquids that each have different:
boiling points
order of steps required for fractional distillation
Boiling flask ➔ fractionating column ➔ condenser ➔ beaker