Pancreatic Enzymes Flashcards
Systematic name of Amylase
Alpha 1,4 glucan-4-glucohydrolase
It is a digestive enzyme that catalyzes the breakdown of starch and glycogen via α, 1-6 branching linkages
Amylase
Major tissue source of Amylase: (2)
Pancreas (acinar cells) & Salivary Glands
Minor Sources of Amylase: (4)
Fallopian tube
Adipose tissue
Small intestine,
Skeletal muscles
Lipase is the smallest, it is the only enzyme that is normally filtered by the renal glomerulus and secreted in urine. Increased amylase in plasma = Increased amylase in urine.
a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.
c. First statement is false, second statement is true.
Diagnostic significance of Amylase: (3)
Acute pancreatitis
Renal failure/disease
Parotitis
Acute pancreatitis, usually seen in men, are caused by: (2)
Overeating
alcohol
Amylase level:
Rise:
Peak:
Normalize:
Rise: 2-12 hours after onset of pancreatitis
Peak: 24 hours
Normalize: 3-5 days
Amylase in urine remains elevated for 1 week
In renal failure, urine amylase increases. Acute pancreatitis attack during deep sleep.
a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.
c. First statement is false, second statement is true.
This is the inflammation of the salivary gland. What is its disease association?
Parotitis.
Associated with mumps.
Amylase isoenzymes: (2)
Salivary amylase (S type) - ptyalin
Pancreatic amylase (P type) - amylopsin
This amylase isoenzyme has greater concentration level and is the major isoenzyme for amylase.
Pancreatic amylase (P type) - amylopsin
Fast moving type of amylase isoenzyme
Salivary amylase (S type) - ptyalin
Enzyme involved in Involved in carbohydrates (polysaccharides) digestion & absorption
Amylase
Slow moving and less anodic type of amylase isoenzyme
Pancreatic amylase (P type) - amylopsin
Amylase Methodologies: (4)
Amyloclastic
Saccharogenic
Chromogenic
Continuous Monitoring
Amylase Methodology:
Measures the disappearance of starch substrate
Amyloclastic
Identify which Amylase Methodology:
Starch-Iodine Complex (Dark-blue) → decrease in
color intensity
Amyloclastic
In Amyloclastic method, the activity of amylase is inversely proportional to absorbance of the test. In Saccharogenic method, the measurement of the reducing sugar (product) is directly proportional to the activity of amylase to polysaccharide substrate
a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.
a. First statement is true, second statement is true.
If glycogen-iodine complex is used instead of starch, what color does the glycogen substrate produce?
Mahogany brown
What is the iodine used for in amyloclastic method?
Iodine is used to stain polysaccharides, which produces dark blue color and decreases as the starch is degraded into simpler form.
Amylase Methodology:
Measures the appearance of the product
Saccharogenic
Amylase Methodology:
Measures the increasing color from production of
product - chromogenic dye fragment
Chromogenic
Identify which amylase methodology:
Starch → reducing sugars
Saccharogenic
