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cee cee 2 finals > Pancreatic Enzymes > Flashcards

Pancreatic Enzymes Flashcards

1
Q

Systematic name of Amylase

A

Alpha 1,4 glucan-4-glucohydrolase

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2
Q

It is a digestive enzyme that catalyzes the breakdown of starch and glycogen via α, 1-6 branching linkages

A

Amylase

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3
Q

Major tissue source of Amylase: (2)

A

Pancreas (acinar cells) & Salivary Glands

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4
Q

Minor Sources of Amylase: (4)

A

Fallopian tube
Adipose tissue
Small intestine,
Skeletal muscles

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5
Q

Lipase is the smallest, it is the only enzyme that is normally filtered by the renal glomerulus and secreted in urine. Increased amylase in plasma = Increased amylase in urine.

a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.

A

c. First statement is false, second statement is true.

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6
Q

Diagnostic significance of Amylase: (3)

A

Acute pancreatitis
Renal failure/disease
Parotitis

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7
Q

Acute pancreatitis, usually seen in men, are caused by: (2)

A

Overeating
alcohol

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8
Q

Amylase level:

Rise:
Peak:
Normalize:

A

Rise: 2-12 hours after onset of pancreatitis

Peak: 24 hours

Normalize: 3-5 days

Amylase in urine remains elevated for 1 week

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9
Q

In renal failure, urine amylase increases. Acute pancreatitis attack during deep sleep.

a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.

A

c. First statement is false, second statement is true.

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10
Q

This is the inflammation of the salivary gland. What is its disease association?

A

Parotitis.

Associated with mumps.

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11
Q

Amylase isoenzymes: (2)

A

Salivary amylase (S type) - ptyalin
Pancreatic amylase (P type) - amylopsin

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12
Q

This amylase isoenzyme has greater concentration level and is the major isoenzyme for amylase.

A

Pancreatic amylase (P type) - amylopsin

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13
Q

Fast moving type of amylase isoenzyme

A

Salivary amylase (S type) - ptyalin

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14
Q

Enzyme involved in Involved in carbohydrates (polysaccharides) digestion & absorption

A

Amylase

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15
Q

Slow moving and less anodic type of amylase isoenzyme

A

Pancreatic amylase (P type) - amylopsin

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16
Q

Amylase Methodologies: (4)

A

Amyloclastic
Saccharogenic
Chromogenic
Continuous Monitoring

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17
Q

Amylase Methodology:

Measures the disappearance of starch substrate

A

Amyloclastic

18
Q

Identify which Amylase Methodology:

Starch-Iodine Complex (Dark-blue) → decrease in
color intensity

A

Amyloclastic

19
Q

In Amyloclastic method, the activity of amylase is inversely proportional to absorbance of the test. In Saccharogenic method, the measurement of the reducing sugar (product) is directly proportional to the activity of amylase to polysaccharide substrate

a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.

A

a. First statement is true, second statement is true.

20
Q

If glycogen-iodine complex is used instead of starch, what color does the glycogen substrate produce?

A

Mahogany brown

21
Q

What is the iodine used for in amyloclastic method?

A

Iodine is used to stain polysaccharides, which produces dark blue color and decreases as the starch is degraded into simpler form.

22
Q

Amylase Methodology:

Measures the appearance of the product

A

Saccharogenic

23
Q

Amylase Methodology:

Measures the increasing color from production of
product - chromogenic dye fragment

A

Chromogenic

24
Q

Identify which amylase methodology:

Starch → reducing sugars

A

Saccharogenic

25
Q

Identify which amylase methodology:

Insoluble starch dye → Soluble starch dye
fragments

A

Chromogenic

25
Q

Insoluble starch dye → Soluble starch dye
fragments

A

Chromogenic

26
Q

Amylase Methodology:

Coupling of several enzyme system to monitor
amylase activity

A

Continuous Monitoring

27
Q

Enumerate the secondary/coupling enzyme involved in Continuous Monitoring Method of Amylase: (3)

A

a-glucosidase
Hexokinase
Glucose-6-phosphate dehydrogenase

28
Q

Systematic name: Triacylglycerol acyl hydrolase

A

Lipase

29
Q

A digestive enzyme that hydrolyzes the ester linkages of fats to produce alcohols and fatty acids. Targets the ester bonds in fat.

A

Lipase

30
Q

Major tissue source of Lipase:

A

Pancreas

31
Q

Pancreatic enzyme that is not affected by the renal disorders and salivary gland diseases

A

Lipase

32
Q

Pancreatic enzyme that is a larger molecule and remains in circulation for 7 days

A

Lipase

33
Q

Enzyme responsible for the hydrolysis of dietary triglycerides in the intestine to 2-monoglyceride and fatty acids

A

Lipase

34
Q

Pathways of Lipid Metabolism: (4)

A

1- Intestinal Absorption Pathway: where pancreatic
lipase is involved
2- Exogenous Pathway
3- Endogenous Pathway
4- reverse Transport Pathway (for cholesterol)

35
Q

Diagnostic Significance of Lipase:

A

Early and specific marker for Acute Pancreatitis

36
Q

Lipase:

Rise
Peak
Normal/Elevated:

A

Rise: 6 hours after the onset

Peak: 24 hours

Elevated: 7 days

37
Q

Assays for Lipase enzyme activity: (2)

A

Cherry Crandall
Tietz

38
Q

Which is the reference method for Lipase Enzyme Activity?

A

Cherry Crandall

39
Q

Assay for Lipase Enzyme Activity:

Substrate:
Titrating Agent:
Endpint

A

Substrate: 50% Olive Oil (triolein)

Titrating Agent: 0.4N NaOH

Endpoint: Fatty acid (Oleic acid)

40
Q

Assay for Lipase Enzyme Activity:

Indicator:
a. Cherry Crandall
b. Tietz

End color
a. Cherry Crandall
b. Tietz

A

Indicator:
a. Cherry Crandall - Phenolphthalein
b. Tietz - Thymolphthalein + Veronal

End color
a. Cherry Crandall - Pink
b. Tietz - Blue

41
Q

Lipase determination methods: (2)

A

Assays (Cherry Crandall, Tietz)
Turbidimetric Method

cee cee 2 finals (5 decks)

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