Liver Enzymes Flashcards
Clinically significant liver enzymes: (3)
- Aspartate Aminotransferase (AST)
- Alanine Aminotransferase (ALT)
- Gamma Glutamyltransferase (GGT)
Aspartate Aminotransferase (AST) is also known as?
Serum Glutamic-Oxaloacetic Transaminase (SGOT)
This enzyme is not liver specific; widely distributed, highest activities in cardiac, liver and skeletal muscle.
Aspartate Aminotransferase (AST)
What are the two isoenzymes of AST?
- Cytoplasmic AST
- Mitochondrial AST
This AST Isoenzyme is the pre-dominant isoenzyme in the serum.
Cytoplasmic AST
This AST Isoenzyme is elevated when there is severe cell membrane damage/injury
Mitochondrial AST
Diagnostic Significance:
After Acute myocardial infarction, AST levels
rise in:________
peaks at: ________
returns to normal in: ________
rise: 6-8 hours
peaks: 24 hours
returns to normal: 5 days
Assay for Enzyme Activity:
This method uses malate dehydrogenase and monitors decrease in absorbance at 340 nm (no
colored product)
Karmen Method
Alanine Aminotransferase (ALT) is also known as?
Serum glutamic-pyruvic transaminase (SGPT)
This enzyme is used to screen blood donors
Alanine Aminotransferase (ALT)
a significant marker in the evaluation of hepatic disorders specific in acute inflammatory disorders
Alanine Aminotransferase (ALT)
used to monitor hepatitis treatment
Alanine Aminotransferase (ALT)
Assay for Enzyme Activity:
Uses Lactate Dehydrogenase and monitors decrease in absorbance at 340 nm
Alanine Aminotransferase (ALT)
This differentiates the cause of hepatic disorder
De Ritis Ratio
AST/ALT ratio
De Ritis ratio where the origin is non-viral
> 1 (AST is Higher)
