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Question 1

OPERATING TEMPERATURE IN CRYOSTAT PROCEDURE

Answer 1

10 to -30 oC

Question 2

OPTIMUM WORKING TEMPERATURE FOR CRYOSTAT PROCEDURE

Answer 2

-18 to -20 oC

Question 3

SPECIMENS FOR -35 oC:

Answer 3

1. fatty tissue
2. skin with fatty subcutis
3. fatty breast
4. omental tissue

Question 4

SEPCIMENS FOR -5 oC to -15 oC

Answer 4

1. BRAIN
2. LYMPH NODES
3. LIVER
4. SPLEEN
5. KIDNEY
6. TESTIS
7. UTERINE
8. CURETTINGS
9. SOFT CELLULAR TUMOR
10. THYROID

Question 5

SPECIMENS FOR -15 to -25 oC

Answer 5

1. muscle
2. connective
3. pancreas
4. uterus
5. cervix
6. non-fatty skin
7. non-fatty breast tissue
8. ovary
9. prostate
10. tongue
11. gut

Question 6

FREEZING METHODS FOR CRYOSTAT METHOD

Answer 6

▪ Liquid nitrogen
▪ Isopentane
▪ CO2
▪ Aerosol spray

Question 7

Allow tissue to equilibrate

Answer 7

LIQUID NITROGEN

Question 8

For histochemistry and intraoperative procedures

Answer 8

LIQUID NITROGEN

Question 9

Fluorinated hydrocarbons

Answer 9

AEROSOL SPRAY

Question 10

MEDIA FOR MOUNTING METHODS IN CRYOSTAT PROCEDURE

Answer 10

➢ Water
➢ 20 to 30% bovine albumin
➢ Von Apathy’s gum syrup
➢ Synthetic water-soluble glycols and resins

Question 11

wave for a few seconds, or fix

immediately in formol-alcohol (15 + 85 ml)

Answer 11

FRESH UNFIXED SECTIONS

Question 12

use albumin or Zwemer’s

chrome-glycerin jelly

Answer 12

FORMALINIZED/FIXED TISSUES

Question 13

TIME FOR PLACING MOUNTED SECTION IN A COUPLIN JAR CONTAINING 40% FORMALDEHYDE

Answer 13

1-5 MINS

Question 14

OPERATION AND MAINTENANCE FOR CRYOSTAT PROCEDURE

Answer 14

▪ Leave ON at all times

▪ Special low-temperature oil should be applied on the microtome