OXPHOS

Question 1

How many electrons do cytochromes carry?

Answer 1

one

Question 2

Iron coordinating porphyrin ring derivatives that act as electron carriers

Answer 2

cytochromes

Question 3

How do cytochrome a, b, and c differ from one another?

Answer 3

Ring additions

Question 4

Iron-sulfur clusters are coordinated by cysteines in the protein. How many electrons do they carry?

Answer 4

one

Question 5

Small, mobile carrier that transfers electrons between the primary dehydrogenases and cytochrome b

Answer 5

Ubiquinone

Question 6

Fully reduced form of ubiquinone

Answer 6

Ubiquinol

Question 7

Shorthand for ubiquinol

Answer 7

QH2

Question 8

Full name of Complex I (electron transport chain)

Answer 8

NADH:ubiquinone oxidoreductase

Question 9

Number of protons transported across membrane by OXPHOS Complex I per NADH

Answer 9

four

Question 10

Full name of Complex II (electron transport chain)

Answer 10

Succinate Dehydrogenase

Question 11

Number of protons transported across membrane by OXPHOS Complex II per NADH

Answer 11

None! Trick question

Question 12

Which enzyme in the electron transport chain is also a part of the TCA cycle

Answer 12

Succinate Dehydrogenase (Complex II)

Question 13

Full name of Complex III (electron transport chain)

Answer 13

Ubiquinone:Cytochrome C Oxidoreductase

Question 14

Soluble heme-containing protein in the intermembrane space, acting as a mobile electron carrier in oxidative phosphorylation

Answer 14

Cytochrome c

Question 15

Cytochrome c carries a single electron between what and what?

Answer 15

From cytochrome bc1 (attached to Complex III) to cytochrome oxidase (Complex IV)

Question 16

The Q cycle in Complex III results in how many electrons transporting how many protons across the membrane?

Answer 16

Two electrons from QH2 end up shuttling four protons across the membrane

Question 17

Full name of Complex IV (electron transport chain)

Answer 17

Cytochrome oxidase

Question 18

Four electrons are used by cytochrome oxidase (complex IV) to do what

Answer 18

reduce one oxygen molecule into two water molecules

Question 19

Complex IV (electron transport chain) pumps how many protons into the intermembrane space per O2 molecule

Answer 19

Four H+

Question 20

Two methods used to feed electrons from NADH from the cytosol into the mitochondria

Answer 20

1) malate-aspartate shuttle

2) glycerol-3-phosphate shuttle (via FADH2)

Question 21

How many ATP are generated per NADH in the electron transport chain?

Answer 21

2.5

Question 22

How many ATP are generated per FADH2 in the electron transport chain?

Answer 22

1.5

Question 23

Three electron transport chain complexes which pump protons to create the gradient

Answer 23

Complexes I, III, IV

Question 24

ATP synthase inhibitor

Answer 24

oligomycin

Question 25

Complex I (OXPHOS) inhibitors

Answer 25

Amytal and rotenone

Question 26

Complex III (OXPHOS) inhibitor

Answer 26

Antimycin A

Question 27

Complex IV (OXPHOS) inhibitors

Answer 27

Cyanide, carbon monoxide, hydrogen sulfide, and sodium azide

Question 28

Lipid soluble weak acids that block oxidative phosphorylation by dissipating the H+ electron gradient. Function as carriers for H+

Answer 28

Uncoupling agents

Question 29

Soluble portion of ATP synthase complex, exposed to matrix and catalyzes the phosphorylation of ADP

Answer 29

F1 subunit

Question 30

Integral, membrane embedded part of the ATP synthase complex. Transports protons form intermembrane space to matrix, dissipating the proton gradient

Answer 30

F0 subunit

Question 31

Antiporter that catalyzes exchange of ADP for ATP across the inner mitochondrial membrane

Answer 31

Adenine Nucleotide Translocase

Question 32

Location of non-shivering thermogenesis

Answer 32

brown adipose tissue

Question 33

Another name for thermogenin, the uncoupling protein seen in non-shivering thermogenesis

Answer 33

UCP1

Question 34

Which contain more mitochondria - white or brown adipose tissue

Answer 34

brown