OxPhos Flashcards
Where does OxPhos happen?
In the IMM, a secondary membrane in the mitochondria that forms cristae. The enzymes are imbedded in the membrane
Where does the mitochondria come from?
The idea is that the mitochondria arrose from endocytosis of a cyanobacteria, as theyposses their own genome, membranes, own prot synthesis mechanism that ressembles prokaryotic, uses fMet (as prokaryotes do), and are affected by several Abx
What is the 2 steps of OxPhos
Well-Ox-transfer of electrons of NADH or FADH2 to O2, translocating protons across the membrane. Phos-the PMF is transolcated back, using the energy to phosphorylate ADP to ATP
So what are the basic enzymes of OxPhos?
NADH Dehydrogenase (complex i), Ubiquinone dehydrogenase (Complex III), cytochrome C oxidase (Complex IV) and succinate dehydrogenase (Complex II)-and carriers_Ubq and CytC
What are the steps of the Ox part of the ETC?
When NADH transfer electrons to CI, which transfers to Ubq, then CIII, then CytC, then CcO, which finally transfers 2e to o2 making H2O - uses the basis REDOX potential
What is the enzyme of the Phos?
F1F0 ATP synthase-made of the F0 is in the membrane, made of the a and c parts (electrons pass through), mouvement is passed down by gamma stalk to alpha and beta subunits (F1) which make ATP