other

Question 1

what are the oris?

Answer 1

pMB1 ori; pUC ori; pSC101 ori; F’ ori; M13 or f1 ori; 2m ori; SV40 ori

Question 2

purpose of pMB1?

Answer 2

has low copy number, about 15-20 copies per cell (used in pBR322-derived plasmids)

Question 3

purpose of pUC ori?

Answer 3

a mutated version of pMB1 that removes control of copy number to yield 500-700 copies per cell

Question 4

purpose of pSC101 ori?

Answer 4

very low copy number, 5 copies/cell

Question 5

purpose of F’ ori?

Answer 5

single copy number 1-2/cell, also encodes partition genes from conjugative plasmid F’; ensures the plasmid will be divided between the cells during replication

Question 6

purpose of M13 or f1 ori?

Answer 6

filamentous phage origin to produce ssDNA which can be packaged into virions

Question 7

purpose of 2micron ori ?

Answer 7

origin from the yeast 2-micron plasmid to allow yeast propagation

Question 8

purpose of SV40 ori?

Answer 8

simian virus 40 origin to allow propagation in some eukaryotic cell lines