enzymes

Question 1

what does exonuc III do?

Answer 1

from e coli, cut single bases off of the 3’ end

Question 2

what does exonuc V do?

Answer 2

can remove bases from both the 5’ and 3’ ends (and has some endo activity as well)

Question 3

T7 Exonuclease

Answer 3

can remove single bases from the 5’ end

Question 4

S1 endonuclease purpose?

Answer 4

from an Aspergillus fungus will cut only single-stranded DNA

Question 5

DNase I purpose?

Answer 5

from cow pancreas will cleave dsDNA, ssDNA and only the DNA in a DNA:RNA hybrid

Question 6

RNAse H purpose?

Answer 6

will cut only RNA in a DNA:RNA hybrid

Question 7

T7 Endonuclease I

Answer 7

will cut at structural oddities like mismatched or cruciform DNA