ospe performed

Question 1

Perform Millon’s test with the given urine sample of a child suffering from
aminoaciduria
a) Record your observation and inference (2 marks)
b) Write the principle of the test (1 mark)
c) Mention the clinical condition where this test will be useful in making a diagnosis

Answer 1

Observation- a red solution or precipitate. (1 mark)
Inference- presence of tyrosine in the given sample. (1 mark)
b) Sodium nitrite reacts with sulphuric acid to form nitrous acid. The phenol group of
tyrosine react with nitrous acid and give red color or precipitate. (1 mark)
c) Tyrosinemia type I, II, III.(1 mark)

modified millions for tyrosine : to 1ml of protein add 10% mercuric sulphate in 10% h2so4 and then add soium nitrate -> red colour

Question 2

Perform an appropriate chemical test to detect the presence of aromatic amino acids in
the given solution. (4)

Answer 2

xanthoprotein test

to 3ml of protein add conc hno3 and boil it later add 90% naoh until the reaction is alkaline
obs yellow to orange

Question 3

Perform a suitable test by using appropriate reagents to detect the presence of αaminoacids in the given sample (

Answer 3

ninhydrin

to 1ml protein add 2 to 3 drops of ninhydrin reagent and the color will change to bluish purple

Question 4

Perform biuret test with given sample
a. Record your observation and inference (1+1)
Observation: Purple color in test tube containing given sample and blue color in the
control tube.
Inference: Presence of peptide linkage
b. Write the principle of the above test? (1)
This test is answered by proteins with two or more peptide linkages.
CuSO4 reacts with peptide link forming co-ordination complex between cupric ions
and the nitrogen atoms of the peptide bonds forming a violet colored complex in
alkaline medium.
c. What is the reacting group? (1mark)

Answer 4

answer is there

Question 5

. Perform a suitable test to detect the presence of creatinine in the given test solution. (

Answer 5

jaffe’s test : to 5ml of creatinine solution add 1ml saturated picric acid solution and 1ml of 10% Naoh
orange colour is obtained
creatinine is confirmed

Question 6

Perform a suitable test to detect the presence of uric acid in the given test solution.

Answer 6

to 3ml of urine add 1ml of benedicts uric acid reagent and 2ml of 20% sodium carbonate solution

blue colour is obtained

uric acid is confirmed. normal urine contains uric acid!!!!

Question 7

Perform Hay’s test in the given urine sample

a) . Record your observation and inference (2)
b) . Write the principle of this test (1)
c) Mention one clinical condition where this test will be useful in making a diagnosis (1)

Answer 7

. Observation – Sulphur powder sinks in the tube containing urine
Inference- Presence of bile salts
b. Bile salts reduce the surface tension leading to sinking of sulphur flowers
c. Obstructive jaundice

hays test sprinkle a little dry sulphur powder on the surface of fresh urine in a test tube taking distilled water as control. if sulphur powder sinks in the presence of bile salts.

Question 8

. Perform Schiffs test with the given sample of urine.

a. Record your observation and inference (2)
b. Write the principle of the test(1)
c. Mention the reference range for serum uric acid

Answer 8

Black precipitate on the filter paper, presence of uric acid

b. Uric acid reduces silver nitrate to metallic silver.
c. Male – 3.5 to 7 mg/dl female- 2.5 to 5 mg/dl

1ml pf uric acid + 1ml of 2% na2co3 + pour rhe sol on filter paper moistened with 1 drop of silver nitrate sol