carbohydrate test

Question 1

what is the test for monosaccharides? MOLISH glucose, fructose

Answer 1

Molish test : add 1-2 drops of molish reagent to 2ml glucose and conc sulphuric acid along the sides.
obs: purple ring

Question 2

benedict’s test ?

Answer 2

to 5ml of benedicts reagent add 8 drops of glucose solution and boil for 2 mins and observe.
different colours orange 1%- 2% and yellow 0.5%-1% green 0.1%-0.5% and red is ablove 2

Question 3

barfoed test for monosacharide

Answer 3

procedure: 2ml of barfoed add 1ml of glucose and boil for 2 mins. cool and add phosphomolybdic acid drop by drop.
brick red in case of mono

Question 4

seliwanoff test

Answer 4

Take two clean, dry test tubes and add 1 ml of the test sample in one test tube and 1 ml of distilled water in another as blank.
Add 2 ml of Seliwanoffs’ reagent to both the test tubes.
Keep both the test tubes in a water bath for 1 min.
Observe the formation of color and note it down.
obs: red colour to denote the presence of ketose sugar