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Order or draw Flashcards

1
Q

what is the order of draw? 12

A
  1. sodium polyanethol sulfonate (sps) (yellow)
  2. sodium citrate tubes (light blue)
  3. serum separator tube (red)
    • plastic:clot activator
    • glass: no additive
  4. serum separatort tube(sst) (gold/mottled red)
  5. rapid serum separator tube (sst)(orange)
  6. heparin:
    • with/out separator gel dark&light greeen
    • with separator gel: light green
  7. EDTA with or without gel separator (lavender/pearly white)
  8. EDTA (pink)
  9. EDTA free of trace elements (royal blue)
  10. EDTA lead free (tan)
  11. Anticoagulant that prevent the deterioration of glucose (grey)
  12. Acid citrate dextrose (acd) (yellow)
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2
Q

what is the first order of draw?

A

sodium polyanethol sulfonate (sps) (yellow)

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3
Q

Which tube has a yellow stopper?

A

Sodium polyanethol sulfonate (sps)

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4
Q

which tube tests blood cultures? bottles

A

Sodium polyanethol sulfonate (yellow top)

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5
Q

how many inversions does sodium polyanethol sulfonate(sps) need?

A

8-10

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6
Q

what is the second order of draw?

A

sodium citrate tubes

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7
Q

what does sodium citrate do?

A

it binds calcium

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8
Q

which tube has a light blue stopper?

A

Sodium citrate tubes

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9
Q

common tests for sps (yellow) tubes

A

blood cultures

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10
Q

what does sodium citrate tubes test for? common tests

A

coagulation test: PT, INR, APTT, D-dimer, fibrinogen, factor assay

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11
Q

how many inversions does a sodium citrate tubes need?

A

3-5

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12
Q

what’s the third order of draw?

A

serum separator
plastic: clot activator
glass: no additive (red)

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13
Q

Which tube has a red stopper?

A

serum separator tube with or without clot activator and separator gel

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14
Q

Common tests for serum separator(red)
plastic: clot activator
glass: no additives

A

Chem: albumin, total protein, ALP, ALT, AST, billirubin,
Serology: ANA, CRP, C3, lyme disease
Endocrine: thyroid, LH, FSH
Thyroid panel: TSH, T4, T3, TIBC
Vitamins: A,D, B

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15
Q

if a serum separator tube (sst) (red, gold, mottled redf) also has a test for cryoglobulins or cold agglutinins what temperature do you keep it at?

A

you prewarm it to body temp and keep it at body temp

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16
Q

how many inversions does a serum separator tube (sst) (red, mottled red, gold) ? 2

A
  • 3-5 for plastic
  • 0 for glass
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17
Q

how long do serum separator tubes need to clot before centrifugation?

A

30 minutes

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18
Q

In plastic serum separator tubes what’s in them?

A

clot activator

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19
Q

what’s in glass serum separator tubes?

A

no additives

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20
Q

common tests for sst tubes (mottled red and gold)

A

same as red serum separator tubes plus the following:
Chemistry: sodium, potassium, magnesium, calcium, carbon dioxide, chloride, BUN, creatinine, total cholesterol, HDL, LDL, triglycerides, HCG

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21
Q

what’s the 4th order of draw?

A

serum separator tubes with clot activator and separator gel

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22
Q

what colors are serum separator tubes with clot activator and separator gel?

A

gold and mottled grey and red

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23
Q

if a serum separator tube, with clot activator and separator gel also has a test for cryoglobulins or cold agglutinins what temperature do you keep it at?

A

prewarm it to body temperature and keep it at body temp

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24
Q

how many inversions are needed for serum separator tubes (sst)

A

3-5

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25
Q

how long do serum separator tubes(sst) (gold, red and grey mottled) need to clot before centrifugation?

A

30 minutes

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26
Q

What’s the 5th order of draw?

A

rapid serum separator tubes (sst)(orange)

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27
Q

what color stoppers are rapid serum separator tubes?

A

orange

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28
Q

What are rapid serum separator tubes?

A

they are the same as serum separator tubes with clot activator and separator gel but they are used for a STAT order

29
Q

how long do rapid serum separator tubes need to clot before cetrifugation?

30
Q

what tubes have any color green stoppers?

A

heparin tubes which prevents thrombin from acting to form fibrin

31
Q

What tests are heparin tubes used for? 4

A
  • STAT chemistry
  • routine chemistry (especially calcium)
  • genetic studies
  • chromosomes
32
Q

what does a light green stoppere mean on a heparin tubes?

A

indicates that the tube has separator gel

33
Q

how many inversions do heparin tubes need?

34
Q

what’s the 7th order of draw?

A

EDTA with or without separator gel

35
Q

what color is EDTA with or without separator gel? 2

A
  • lavender
  • pearly white
36
Q

what tube is lavender or pearly white?

A

EDTA with or without separator gel

37
Q

what does EDTA with or without separator gel test? 2

A

Hematology: CBC, hemoglobin, hematocrit, platelet count, ESR, Factor V, HBA1c, ammonia

38
Q

how does EDTA with or without separator need to be stored?

A

ammonia (on ice)

39
Q

how many inversions does an EDTA tube with or without separator gel need?

40
Q

whats the 8th order of draw?

41
Q

what color is an regular EDTA stopper?

42
Q

which tube has a pink stopper?

43
Q

What is regular EDTA (pink) for?

A
  • blood bank collection (cross matching)
44
Q

how many inversions does EDTA need?

45
Q

what’s the 9th order of draw?

A

EDTA free of trace elements

46
Q

what color stopper is EDTA free of trace elements?

A

royal blue

47
Q

what tube has a royal blue stopper?

A

EDTA free of trace elements

48
Q

what does EDTA free of trace elements test? 3

A
  • trace elements
  • toxicology
  • nutritional tessting
49
Q

how many inversions does EDTA free of trace elements need?

50
Q

what’s the 10th order of draw?

A

lead free EDTA

51
Q

what color is lead free EDTA?

52
Q

which tube has a tan stopper?

A

lead free EDTA

53
Q

what does lead free EDTA test?

A

lead levels

54
Q

how many inve3rsions does lead free EDTA need?

55
Q

what’s the 11th order of draw?

A

anticoagulant that prevents deterioration of glucose

56
Q

what color is the anticoagulant tube?

57
Q

what tube what’s a grey stopper

A

anticoagulant

58
Q

what do anticoagulant that prevents the deterioration og glucose test? 3

A
  • glucose
  • blood alcohol levels
  • lactic acid
59
Q

if testing for lactic acid what do you need to do?

A

put it on ice (ice slurry)

60
Q

how many inversions does an anticoagulant that prevents deterioration of glucose?

61
Q

what’s the 12th order of draw?

A

acid citrate dextrose

62
Q

what color stopper is acid citrate dextrose (acd) tube?

63
Q

what tube has a yellow stopper?

A

acid citrate dextrose

64
Q

what does acid citrate dextrose test?

A

DNA analysis such as paternity testing

65
Q

how many inversions does acid citrate dextrose need?

66
Q

which testing tubes need to be protected from light? 4

A
  • billireuben
  • many vitamins
  • carotene
  • folate
67
Q

which testing tubes need to be kept cold (ice slurry) 4

A
  • ammonia
  • gastrin
  • lactate/ lactic acid
  • pH
68
Q

which testing tubes need to be kept at body temperature? 3

A
  • cold agglutinins=
  • cryoglobulin
  • cryofibrinogen

Phlebotomy (3 decks)

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