order of draw Flashcards
What is first in order of draw?
Sterile
What is the use for sterile collection?
Blood culture specimens in microbiology
Why is sterile first?
Minimize microbial contamination
What is second in order of draw?
Blue top (Sodium)
What is the use for the sodium collection labs?
Coagulation determination
Why is blue top (sodium) second?
All other additives affect coagulation tests
How many times do you invert sterile draws?
8-10
How many times do you invert blue top9sodium) draws?
3-4
What is third in order of draw?
Red Serum tubes with or without separator gel
What is the use for red serum tubes?
Serum determination in chemistry, used for diagnostic testing for infectious disease
How long must the red serum tubes clot for ?
30-60 minutes
Why is red serum tubes third?
Can affect coagulation
How many times must you invert your red serum tubes?
5
What is fourth in order of draw?
Yellow/tiger serum tubes with or without separator gel
What is the use for yellow/tiger serum tubes?
Serum determination in chemistry
How long must the yellow/tiger serum tubes clot?
30 minutes
Why is yellow/tiger serums fourth?
can affect coagulation test, anticoagulants in other tubes will override thrombin or silica
What is fifth in order of draw?
light green/army green heparin with or without gel
What is the use for light green/army green heparin tubes?
Whole blood or plasma determination in chemistry
Why is light green/army green heparin fifth?
Affects coagulation test, interferes with serum collection
How many times must you invert yellow/tiger serum tubes?
5-8
How many times must you invert light green/army green heparin tubes?
8
what is sixth in order of draw?
green heparin with or without gel
What is the use for green heparin tubes?
Whole blood or plasma determinations in chemistry
Why are green heparin tubes sixth?
affects coagulation test and serum collection.
How many times must you invert green heparin tubes?
8
What is seventh in the order of draw?
lavender, pink, pearl or tan EDTA tubes
What is the use for lavender EDTA tubes?
whole blood determination sin hematology.
What is the use for pink and pearl EDTA tubes?
routine immunohematology testing for blood donor and screening
What is the use for tan EDTA tubes?
Lead determinations
Why are EDTA tubes seventh?
Responsible for more carryovers than any other additive
How many times must you invert EDTA tubes?
8
What is eighth in order of draw?
Glycolytic inhibitor tubes
What is the use for Glycolytic inhibitor tubes?
glucose determinations
What are the different types of glycolytic inhibitor tubes?
oxalate and sodium fluoride
What is the use for oxalate glycolytic inhibitor tubes?
Gives plasma samples
What is the use for sodium fluoride glycolytic inhibitor tubes?
’
its the antiglycolytic agent
Why is glycolytic inhibitor tubes eighth?
affects sodium and potassium, oxalates damage membranes and can interfere with enzyme reactions
How many times must you invert glycolytic inhibitor tubes?
8
What is the additive for sterile tubes?
Sodium polyanethol sulfonate or SPS
What is the additive for sodium collection labs?
Sodium citrate
What is the additive for red serum tubes?
clot activators like thrombin or silica
What is the additive for yellow/tiger serum tubes?
clot activators like thrombin and silica and thixotropic gel
What is the additive for light green/ army green heparin tubes?
lithium heparin, thixotropic gel
What is the additive for green heparin tubes?
Sodium heparin or lithium heparin
What is the additive for EDTA tubes?
EDTA
What is the additive for glycolytic inhibitor tubes?
antiglycolytic agent like potassium oxalate and sodium fluoride