OPT2222 Exam 1 Flashcards
How do you control microorganisms?
Disinfectants
Antiseptics
Sterilization
Hand Washing (Sanitization and Degermination)
What is sepsis?
State of putrefaction or decay
What is the application of agents onto body surfaces (skin, conjunctiva) to destroy or slow growing microorganisms?
Antiseptics
pH balanced and isotonic
What is the application of agents onto inanimate surfaces (counters, instruments) to destroy or slow growing microorganisms?
Disinfectants
What is the complete removal and destruction of all microorganisms on an inanimate surface?
Sterilization
What microorganisms have the highest microbial resistance?
Nonliving prions
Dormant Bacterial endospores
[things you can’t kill since they aren’t alive]
What microorganisms have moderate microbial resistance?
Pseudomonas Mycobacterium Tuberculosis (b/c of waxy coat) Staphylococcus Aureus (most plentiful microbe on body) Protozoan Cysts (can fight destruction)
What microorganisms have the least microbial resistance?
Bacterial vegetative cells
Fungal spores, hyphae, yeast
Enveloped Viruses
Protozoan Trophozoites
What is microbial death?
Permanent loss of reproductive capability
hard to detect even under optimal growth conditions
What are the 9 factors that influence death rate of microorganisms?
Number of microbes Nature of microbes Temperature pH Concentration of agent Mode of action of agent Presence of solvents Organic Matter Inhibitors
What is the anitmicrobial mode of action of drugs, detergents and alcohol?
Cell wall becomes fragile and cell lyses
remember….eukaryotic cells don’t have a cell wall
What is the anitmicrobial mode of action of detergent surfactants?
Cell membrane loses integrity (surface wall breaks up)
What is the anitmicrobial mode of action of cholramphenicol, ultraviolet radiation and formaldehyde?
Prevention of replication, transcription, translation and protein synthesis
What is the anitmicrobial mode of action of heat?
Disrupt or denature proteins
What is the shortest time to kill all microbes at a specific temperature?
Thermal Death Time (TDT)
What is the lowest temperature required to kill all microbes in 10 minutes?
Thermal Death Point (TDP)
What is moist heat sterilization?
Steam under pressure
Autoclave
Steam must reach surface of item being sterilized
Item must not be heat, pressure or moisture sensitive
What are the requirements for autoclaving?
15 psi
121 degrees Celsius
10-40 minutes
What method can be used for intermittent sterilization when substances cannot withstand autoclaving?
Tyndallization (nonpressurized application of steam)
not effective in medicine
What is tyndallization used for?
canned foods and laboratory media
What is deep penetrating energy that causes electrons to leave their orbit (breaks/damages DNA)?
Ionizing Radiation (gamma, x-rays, cathode ray)
How many levels of chemical decontamination and what are they?
3 levels:
High-level germicides
Intermediate level
Low-level
What do high level germicides do and what are they used for?
They kill endospores (sterilants) and are used in sterile environments (body tissue).
They are usually toxic.
What do intermediate level germicides do and what are they used for?
They kill fungal spores, tubercle bacillus and viruses. They are used to disinfect devices in contact with mucous membranes.
What do low level germicides do and what are they used for?
They eliminate vegetative bacteria, vegetative fungal cells and sensitive viruses. They are used to clean surfaces that touch skin.
How do halogens work?
Denature proteins by disrupting disulfide bonds
How do surfactants work?
Dissolve membrane lipids and coagulating proteins of vegetative bacterial cells and fungi
Nosocomial or HAI
hospital or health care center acquired infection
Zoonosis
animal acquired infection in a human
hypopyon
accumulation of pus in anterior chamber of eye
What does the Greek word pathos mean?
Feeling, suffering
What does the Greek word logia mean?
The study of
What does pathology mean?
The study of disease
What are the top 5 causes of Blindness in the US?
- Age-related macular degeneration
- Cataracts (top cause worldwide)
- Diabetic retinopathy
- Glaucoma
- Corneal opacification
How many people get regular eye exams?
21% of population
What is the most common differential diagnosis made?
Diagnosis of the Red Eye
What are Cataracts?
A clouding of the natural lens of the eye
Only solution is surgery
What is involved in cataract surgery?
Microincision
Phacoemulsification - ultrasonic, breaks up cataract and removes through same tool
Femtosecond Laser Surgery - makes incision and breaks up cataract, removal and insertion of IOL still done mechanically
Removal of clouded lens
Insertion of IOL
What are the goals of microsurgery of the eye?
Improve vision for the visually impaired
Restore vision for those who cannot see
What is glaucoma?
An increase in the eye pressure (normal is 18-20 mmHg) that damages the optic nerve.
Though pressure is not the only cause and is not always directly related to damage)
How many people are affected by glaucoma?
67 million worldwide
3 million in US
1 out of 40 are unaware have it (happens gradually, painless)
African Americans are 4X as common and 10X as likely to go blind.
What do you lose first with glaucoma?
peripheral vision
How is glaucoma diagnosed?
Measure eye pressure
Image the Optic nerve
Optical Coherence Tomography (OCT)
Measure Visual Field
What are the diseases of the Retina?
Age-related macular degeneration (ARMD)
Diabetic retinopathy
What does the Greek word pharmakon mean?
Poison or Drug
What is pharmacology?
The study of drugs or medicinals
How are ocular drugs administered?
Topical
Oral - very difficult
Injection under or into eye
Intravenous
What are Water mixtures?
Substances that are physically but not chemically combined
Water comprises how much of body weight?
50-75%
The eye is mostly water.
What is solvency?
The ability to dissolve chemicals
Do hydrophobic or hydrophilic substances dissolve in water?
Hydrophilic (charged substances) dissolve in water.
Hydrophobic (neutral substances) dissolve in oil.
What are the different types of drugs?
Solutions Suspensions Colloids Emulsions Ointments Creams