Old Exam Questions Flashcards
LAB are predominantly which genus?
Lactobacillus spp.
True or False. Lower the pH, higher energy is required by cell to maintain cell intracellular pH neutrality and less energy is left for the growth.
True
How many hours does milk need to cool down to 4 - 7 C?
2 hours
Which pathogen dominates in fish and shellfish?
Vibrio
True or False. Lactic acid bacteria are always a good and positive bacteria to have in your dairy products?
False.
Most common minimum Aw for mold
0.8
Which will require the highest “intervention” dose for inactivation?
Clostridium spore
What does VBNC stand for?
Viable but not culturable
What does temperature regulate?
The growth of bacteria
The lipid content of the cell membrane
Gene expression of bacteria
Explain the how and why ground meat products are more correlated to increases in outbreaks.
Ground meat products are correlated to increase outbreaks given the composition of low quality meat and improper cooking/handling.
What bacteria are apart of the spoilage of fish and shellfish products
Pseudomonas
Vibrio
Morganella spp.
True or False. Common microorganisms and foodborne pathogens that are present on food (e.g., beef) surfaces show seasonal variation
True
Coliforms ferment lactose to produce?
CO2
At what stage would bacteria produce their byproducts (toxins, enzymes, etc)
Late log phase
True or False. Do microorganisms need both processes of adapting to acid protection in order to survive?
True
What is the most common minimum Aw for yeast?
0.80
Mold is able to survive low Aw because?
Compatible solutes
Psychotropic bacteria that cause sweet curdling do so by attacking?
Casein
Stickiness on meat starts at what CFU?
10^8 CFU/cm^2
What is the most important bacterial foodborne pathogen that can multiply in raw milk stored at low temperatures and can cause foodborne outbreaks?
Listeria monocytogenes
Food spoilage includes
Reduction of the texture of food
It can be harmful to the consumer
Reduction of the nutritional value of food
Reduction of the flavor of food
What is biofilm?
Aggregation of bacteria living together on a solid surface.
True or False.The minimum Aw of bacteria, mold, and yeast to grow is the same as the minimum Aw for them to produce toxins.
False
Minimum water activity needed for the growth of gram-negative and gram-positive bacteria.
0.97: 0.9, respectively
What is one of the main specific issues with leaving fish for too long at improper temperature conditions?
Histamines
What is infection and intoxication?
Ingestion of organism grow, replicate, colonize, and potentially release toxins in the host’s intestine; Ingestion of bacteria that was growing on food and releasing toxins, respectively
What is the purpose of pasteurization?
Reduce microbial load and enzyme activity
pH levels of meat from a well rested animal is
5.4-5.6
The Most important psychrotrophic bacteria in milk that can multiply at low temperatures and can result in milk spoilage
Pseudomonas
The application of two or more hurdles to control the growth of foodborne pathogens facilitates
- It can be more effective than one intervention treatment
- Facilitates application of milder intervention treatment
- Helps retain organoleptic properties of food after intervention
Proteolysis produce?
Bitter, putrid flavor and coagulation
Major agents of dairy spoilage?
LAB
Coliforms
Psychotropic bacteria
Which milk protein is more susceptible to the susceptible microbial proteolysis?
Casein
A main byproduct of spoilage bacteria on fish is an enzyme. What is the main issue with these bacteria and enzyme?
Even if the bacteria is killed the enzyme can still be active
True or False. At low cellular pH, cells have to spend more energy to maintain neutrality and less energy is left for their growth.
True
Common source of spoilage bacteria in meat products is
Hide, hooves, feathers
Sources of contamination of raw milk
Soil, skin tissue, water quality and utensils
Sweet Curding is predominately caused by which genus?
Bacillus spp.
Lipolysis produce?
Rancid and fruit flavors
True or False. Biofilms are more difficult to get rid of compare to bacteria.
True
What methods of growth are most likely to be related to Psychrotropic Bacteria?
Proteases and lipases
What is NOT an intrinsic factor? water activity, temp, naturally occurring compounds, pH, nutrients
Temp
What is the primary energy source found in milk?
Lactose
Why is milk a good growth media?
neutral pH, sugar, high water content, protein, fat
At neutral pH which will response/mechanism will be turned on?
Homeostatic response
Modified Atmosphere Packaging (MAP) works by increasing the concentration of
CO2
What is the source of microorganisms in beef?
Hair
hooves
hide
GI Tract
What are the USDA and FDA responsible, respectively?
Meat and poultry and all other food products, respectively
What is the purpose of the FoodNet?
Designed to monitor each of the events that occur along the foodborne diseases
True or False. Aerobic and anaerobic pathways are both high energy productions
True
Why does the Emetic toxin (Cereulide) have such a fast onset for illness?
The toxin is an amino acid synthetase
True or False. Only coagulase-positive Staphylococcus aureus strains are pathogenic
False
Bacillus is a….
Gram-positive, spore-forming, rod-shaped
Diarrheal Food Poisoning and Emetic Food Poisoning by B. cereus are commonly from which sources?
Fish and meats; Starchy food (rice), respectively
What is the most distinct symptom of Emetic syndrome associated with a cereulide -producing Bacillus strain?
Vomiting
Fermentation of vegetable is a way of preserving food and its nutritive values. Which microorganism they use also depends on their culture. What are they?
America/Europe: LAB and Yeast
Asia: Mold
What are Indicator Organisms used to assess?
Food safety and food quality
Extracellular protease acts on casein molecules resulting in free amino acids and peptides. What are they used for or cause?
1) Required for the growth of many LABs
2) Flavor development during ripening process of cheese
3) Generation of either bitter peptides or non-bitter peptides
Heterofermentation are responsible for?
Aroma and flavor
Emetic Food Poisoning is caused by
Cereulide
Which Bacillus species has the ability to grow in the cold (below 7C)?
Bacillus weihenstephanensis
What are some of the reasons that we ferment foods?
We ferment foods due to climate, food preservation, digestibility of grains and flavor/aroma.
Which fermentation pathway is the most efficient and common?
Embden-Meyerhof-Parnas
What are Index organism and Indicator organism?
Microorganism or group of microorganisms that is indicative of a specific pathogen; Microorganism or group of microorganisms that are indicative that a food has been exposed to conditions that pose an increased risk, but not necessarily pathogenic, respectively
Sugar is converted to what product(s) during fermentation?
CO2
Acetic acid
Lactic Acid
Ethanol
Homofermentation are responsible for?
Acid production
What is mandatory criteria?
A limit which may not be exceeded. If exceeded food need to destroyed, reprocessed, diverted.
Staphylococcal toxin
Highly resistant to proteolytic enzymes
Heat-stable
Common reservoir of S. aureus?
Hair, nose, respiratory droplets, skin
Which factors determine which pathway organism uses
Oxygen conditions
Availability of sugar e.g. pentoses, hexoses
Genetic make up
Membrane proteins
True or False. B. cereus outbreaks are highly underestimated.
True